Rodríguez-Morató, Jose, 1987-Jayawardene, SarahHuang, Neil K.Dolnikowski, Gregory G.Galluccio, Jean M.Lichtenstein, Alice H.Matthan, Nirupa R.2020-12-112020Rodríguez-Morató J, Jayawardene S, Huang NK, Dolnikowski GG, Galluccio J, Lichtenstein AH, Matthan NR. Simplified method for the measurement of plasma alkylresorcinols: Biomarkers of whole-grain intake. Rapid Commun Mass Spectrom. 2020; 34(14):e8805. DOI: 10.1002/rcm.88050951-4198http://hdl.handle.net/10230/45978Rationale: Consumption of whole grains is negatively associated with cardiovascular disease (CVD) risk but quantification of whole-grain intake is challenging. Alkylresorcinols (ARs) are biomarkers of whole-grain intake. Current methods for AR quantification involve a time-consuming multi-step separation process that hampers applicability in large-scale studies. Methods: We developed a streamlined method to quantify ARs in human plasma based on protein precipitation and direct injection into an ultra-high-performance liquid chromatograph coupled to a quadrupole time-of-flight mass spectrometer operating in atmospheric pressure chemical ionization negative ion mode. Results: Separation of five major ARs was achieved, with linearity in the 5 to 550 nmol/L range and a lower limit of detection (LOD) of 0.5 nmol/L and quantification (LOQ) of 5 nmol/L. The within-run and between-run precision and accuracy were below 15%, and recoveries above 90%. Once validated, the method was applied to measure concentrations of plasma ARs in subjects who participated in a randomized, crossover trial evaluating the effect of carbohydrate type on CVD risk factors. The unrefined carbohydrate diet with the highest fiber content resulted in the highest plasma AR concentration (93 ± 78 nmol/L), and was significantly different (p <0.01) from lower fiber diets (18 ± 26 nmol/L and 19 ± 26 nmol/L, simple and unrefined carbohydrate, respectively). Conclusions: This method offers a simplified approach to measure concentrations of plasma ARs as an objective biomarker of whole-grain intake that can be applied to large-scale cohort studies.application/pdfengThis is the peer reviewed version of the following article: Rodríguez-Morató J, Jayawardene S, Huang NK, Dolnikowski GG, Galluccio J, Lichtenstein AH, Matthan NR. Simplified method for the measurement of plasma alkylresorcinols: Biomarkers of whole-grain intake. Rapid Commun Mass Spectrom. 2020; 34(14):e8805. DOI: 10.1002/rcm.8805, which has been published in final form at http://dx.doi.org/10.1002/rcm.8805. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/rcm.8805AlkylresorcinolsBiomarkersLiquid chromatographyRandomized clinical trialWhole-grainsinfo:eu-repo/semantics/openAccess