Wotton, Karl R.Jiménez Guri, EvaCrombach, AntonCicin Sain, DamjanJaeger, Johannes, 1973-2015-11-192015-11-192015Wotton KR, Jiménez-Guri E, Crombach A, Cicin-Sain D, Jaeger J. High-resolution gene expression data from blastoderm embryos of the scuttle fly Megaselia abdita. Scientific data. 2015;2:150005. DOI: 10.1038/sdata.2015.52052-4463http://hdl.handle.net/10230/25161Gap genes are involved in segment determination during early development in dipteran insects (flies, midges, and mosquitoes). We carried out a systematic quantitative comparative analysis of the gap gene network across different dipteran species. Our work provides mechanistic insights into the evolution of this pattern-forming network. As a central component of our project, we created a high-resolution quantitative spatio-temporal data set of gap and maternal co-ordinate gene expression in the blastoderm embryo of the non-drosophilid scuttle fly, Megaselia abdita. Our data include expression patterns in both wild-type and RNAi-treated embryos. The data-covering 10 genes, 10 time points, and over 1,000 individual embryos-consist of original embryo images, quantified expression profiles, extracted positions of expression boundaries, and integrated expression patterns, plus metadata and intermediate processing steps. These data provide a valuable resource for researchers interested in the comparative study of gene regulatory networks and pattern formation, an essential step towards a more quantitative and mechanistic understanding of developmental evolution.application/pdfeng© This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.Insectes -- Genèticainfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/sdata.2015.5info:eu-repo/semantics/openAccess