Grey, CorinneClément, Julie A.J.Buard, JeromeLeblanc, BenjaminGut, Ivo GlynneGut, MartaDuret, Laurentde Massy, Bernard D.2018-06-142018-06-142017Grey C, Clément JA, Buard J, Leblanc B, Gut I, Gut M et al. In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites. Genome Res. 2017 Apr;27(4):580-90. DOI: 10.1101/gr.217240.1161088-9051http://hdl.handle.net/10230/34894In mouse and human meiosis, DNA double-strand breaks (DSBs) initiate homologous recombination and occur at specific sites called hotspots. The localization of these sites is determined by the sequence-specific DNA binding domain of the PRDM9 histone methyl transferase. Here, we performed an extensive analysis of PRDM9 binding in mouse spermatocytes. Unexpectedly, we identified a noncanonical recruitment of PRDM9 to sites that lack recombination activity and the PRDM9 binding consensus motif. These sites include gene promoters, where PRDM9 is recruited in a DSB-dependent manner. Another subset reveals DSB-independent interactions between PRDM9 and genomic sites, such as the binding sites for the insulator protein CTCF. We propose that these DSB-independent sites result from interactions between hotspot-bound PRDM9 and genomic sequences located on the chromosome axis.application/pdfeng© 2017 Grey et al.; Published by Cold Spring Harbor Laboratory Press. This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1101/gr.217240.116Protein bindingPrdm9 bindingDna double-strand breaksinfo:eu-repo/semantics/openAccess