González Medina, Alberto, 1990-Pazo Pelegrí, Esther, 1993-Hidalgo Hernando, ElenaAyté del Olmo, José2022-01-202021González-Medina A, Pazo E, Hidalgo E, Ayté J. SWI/SNF and RSC remodeler complexes bind to MBF-dependent genes. Cell Cycle. 2021;20(24):2652-61. DOI: 10.1080/15384101.2021.20082031538-4101http://hdl.handle.net/10230/52267In fission yeast, MBF-dependent transcription is required for cells to complete S phase. The MBF transcription factor is regulated through a complex feedback mechanism that involves the co-repressors Yox1 and Nrm1 that are loaded onto MBF at the end of S phase, while positive transactivation is achieved through the constitutive binding of the co-activator Rep2. Here we show that Rep2 is required to fully recruit the chromatin remodelers SWI/SNF and RSC to MBF-regulated promoters. On the contrary, Nrm1 and Yox1, when bound to the MBF complex, block the approximation of these chromatin remodelers to MBF-regulated promoters. We propose that SWI/SNF and RSC are recruited to MBF-regulated genes, and RSC together with SAGA complex are important to regulate the G1-to-S transcriptional wave. Mutants of these remodeler complexes are highly sensitive when cells are exposed to insults that challenge DNA synthesis.application/pdfeng© This is an Accepted Manuscript of an article published by Taylor & Francis in Cell cycle on 2021, available online: http://www.tandfonline.com/10.1080/15384101.2021.2008203.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/15384101.2021.2008203MBFRSC complexStartinfo:eu-repo/semantics/openAccess