Identifying the factors that influence the stability of the embryo culture system and the sensivity of the Mouse Embryo Assay : The critical role of oil in the IVF lab

Mestres Gonzalvo, Enric

Identifying the factors that influence the stability of the embryo culture system and the sensivity of the Mouse Embryo Assay : The critical role of oil in the IVF lab

Document Type

Doctoral thesis
Published Version

Author

Mestres Gonzalvo, Enric

Director

Costa Borges, Nuno Luis

Publication Date

2022-02-18

Pages

234 p.

Citation

Mestres Gonzalvo, E. Identifying the factors that influence the stability of the embryo culture system and the sensivity of the Mouse Embryo Assay : The critical role of oil in the IVF lab. 2022. handle: http://hdl.handle.net/10803/673533

This citation was generated automatically.

Doctoral program

Programa de doctorat en Biomedicina

Abstract

The embryo culture system is composed by all those elements with the potential to impact embryo development. Its optimization is required to stabilize in vitro embryo culture and produce reliable and reproducible results in the IVF and QC laboratories. In this thesis we have shown how several components of the culture system, including but not limited to the mineral oil viscosity and volume, the relative humidity in the incubators, the culture dish design, and the type of medium and protein supplementation, are key elements to stabilize the culture conditions. In addition, we have developed a specialized Mouse Embryo Assay protocol to detect peroxide-related toxicity in mineral oil samples, and shown its effectiveness to screen multiple commercial oils used in human IVF. As a whole, the results obtained in this thesis could help to establish regulated and standardized methodologies for the future of QC testing in the IVF field.
El sistema de cultiu embrionari està compost per tots aquells elements amb el potencial d’afectar al desenvolupament embrionari. La seva optimització és necessària per estabilitzar el cultiu embrionari in vitro i generar resultats fiables i reproduïbles als laboratoris de FIV i Control de Qualitat. En aquesta tesi hem demostrat com diversos components del sistema de cultiu, inclosos però no limitats a la viscositat i el volum d’oli mineral, la humitat relativa a dins dels incubadors, el disseny de les plaques de cultiu, i el tipus de medi i suplement proteic, són elements essencials en l’estabilització de les condicions de cultiu. Així mateix, hem desenvolupat un protocol especialitzat de l’Assaig amb Embrions de Ratolí per detectar toxicitat causada per peròxids en mostres d’oli mineral, i hem confirmat la seva efectivitat pel cribratge de varis olis comercials utilitzats en FIV humana. En conjunt, els resultats presentats en aquesta tesi podrien esdevenir un primer pas cap a la regulació i estandardització de les

Keywords

Quality control IVF, Embryology, Mineral oil, In vitro culture conditions, Control de qualitat, FIV, Embriologia, Oil mineral, Condicions de cultiu in vitro