The early development of the inner ear: the role of notch and fgf pathways in aerly otic neural versus non-neural patterning

Abelló Sumpsi, Gina

The early development of the inner ear: the role of notch and fgf pathways in aerly otic neural versus non-neural patterning

Document Type

Doctoral thesis
Published Version

Author

Abelló Sumpsi, Gina

Director

Alsina i Español, Berta
Giráldez, Fernando

Publication Date

2008-01-25

Citation

Abelló Sumpsi, G. The early development of the inner ear: the role of notch and fgf pathways in aerly otic neural versus non-neural patterning. Universitat Pompeu Fabra; 2008. handle: http://hdl.handle.net/10803/7121

This citation was generated automatically.

Doctoral program

Programa de doctorat en Biomedicina

Abstract

Otic neuronal precursors are specified in the otic placode but interestingly do so only in the anterior domain of the otic placode, the proneural domain. In the present study, we have explored why only this territory has the competence to undergo neurogenesis, this means the early events of otic proneural regionalization and neural commitment. The proneural and non-neural domains presented complementary gene expression patterns of transcription factors and members of the Notch pathway. Overall, we propose that proneural character is acquired in the anterior territory by the action of localized ectodermal FGF8-FGF10 signaling that enhances Sox3 function. FGF signals through Sox3 activity would be essential for the specification of the proneural domain versus a non-neural territory, while Notch would be involved in refining this early regionalization.
Els precursors neurals de la oïda són especificats dins un subdomini de la placoda acústica, el domini proneural. En aquesta tesis doctoral, s'ha explorat la raó per la qual la competència neural només és adquirida en aquest subdomini. Els dominis neural i no-neural del territori presenten dominis d'expressió genètica complementaris. Aquest treball proposa que el caràcter proneural és adquirit en el subdomini anterior del territori per l'acció de FGF8 i FGF10 localitzats en lï¿½ectoderm ï¿½tic que promouen lï¿½acciï¿½ de Sox3. De manera que, les senyals FGF i lï¿½activitat Sox3 sï¿½n essencials per lï¿½especificaciï¿½ del domini proneural, mentre que la via Notch ï¿½s necessï¿½ria per refinar la regionalitzaciï¿½ final entre un domini neural complementari a un domini no-neural.

Keywords

ratolí, peix, pollet, embrió, cultius d'embrió, electroporació, marcatge cellular, Sox3, resctricció de llinatges, Notch, FGF, territori no-neural, neurona, territori proneural, regionalització, oída, Desenvolupament, lineage restrictions, embryo culturing, cell labelling, electroporation, DiI, mouse, zebrafish, chick, embryo, otic placode, neurogenesis, DAPT, Sox3, patterning, signaling, FGF, proneural, Notch, Development