Temperature control for cell division : from microfluidics to AI

Salaverria Izaguirre, Pablo G.

Temperature control for cell division : from microfluidics to AI

Document Type

Doctoral thesis
Published Version

Author

Salaverria Izaguirre, Pablo G.

Director

Vernos, Isabelle
Cramer, Jeremy

Publication Date

2019-11-22

Pages

172 p.

Citation

Salaverria Izaguirre, P. Temperature control for cell division : from microfluidics to AI. Universitat Pompeu Fabra; 2019. handle: http://hdl.handle.net/10803/668014

This citation was generated automatically.

Doctoral program

Programa de doctorat en Biomedicina

Abstract

This PhD thesis aimed to understand the impact of temperature at different levels (biology to physics) while performing live-cell imaging. A study and characterization of the role of the temperature during the cell division while using live-cell imaging on several biological models was done. Microfluidics was used to study it while accurately controlling the temperature on a microscope stage. Followed by characterizing a microscope setup to quantify the impact of different external factors on the sample temperature. Based on that, I built new microfluidic based devices able to control the sample temperature with high accuracy. Those devices were designed, manufactured and validated in collaboration with four research teams that used different biological models: S. Pombe, Hela-Kyoto cells, kidney cancer spheroids and in vitro microtubules. Finally, I developed an AI-based image classifier to study cell division, using data from samples thermalized by the accurate temperature control devices described above.
Esta tesis doctoral tuvo como objetivo comprender el impacto de la temperatura en diferentes niveles (biología o física) al realizar microscopia. Se estudio y se caracterizó la implicación de la temperatura aplicada la división celular de distintos modelos biológicos utilizando microscopia. Se utilizo la microfluidica para estudiarlo mientras se controlaba con precisión la temperatura en un microscopio. Se caracterizo un set de microscopia para cuantificar el impacto de diferentes factores externos en la temperatura de la muestra. Con ello, construí nuevos dispositivos microfluidicos capaces de controlar la temperatura de la muestra con precisión. Esos dispositivos fueron diseñados, fabricados y validados en colaboración con cuatro equipos de investigación que utilizaron diferentes modelos biológicos: S. Pombe, células Hela-Kyoto, esferoides de cáncer de riñón y microtúbulos in vitro. Finalmente, desarrolle un clasificador de imágenes usando inteligencia artificial para estudiar la división celular, utilizando imágenes adquiridas con el preciso control térmico descrito anteriormente.

Keywords

Temperature control, Live-cell imagine, Microfluidics, Cell division and Artificial Intelligence, Control de temperatura, Imagen células en vivo, Microfluidica, División celular e inteligencia artificial