Mechanism of regulation of the RPL30 pre-mRNA splicing in yeast

Macías Ribela, Sara

Mechanism of regulation of the RPL30 pre-mRNA splicing in yeast

Document Type

Doctoral thesis
Published Version

Author

Macías Ribela, Sara

Director

Vilardell, Josep

Publication Date

2008-06-13

Citation

Macías Ribela, S. Mechanism of regulation of the RPL30 pre-mRNA splicing in yeast. Universitat Pompeu Fabra; 2008. handle: http://hdl.handle.net/10803/7112

This citation was generated automatically.

Doctoral program

Programa de doctorat en Biomedicina

Abstract

The mechanisms of pre-mRNA splicing regulation are poorly understood. Here we dissect how the Saccharomyces cerevisiae ribosomal L30 protein blocks splicing of its pre-mRNA upon binding a kink-turn structure including the 5' splice site. We show that L30 binds the nascent RPL30 transcript without preventing recognition of the 5' splice site by U1 snRNP but blocking U2 snRNP association with the branch site. Interaction of the factors BBP and Mud2p with the intron, relevant for U2 snRNP recruitment, is not affected by L30. Furthermore, the functions of neither the DEAD-box protein Sub2p in the incipient spliceosome, nor of the U2 snRNP factor Cus2p on branch site recognition, are required for L30 inhibition. These findings contrast with the effects caused by binding a heterologous protein to the same region, completely blocking intron recognition. Collectively, our data suggest that L30 represses a spliceosomal rearrangement required for U2 snRNP association with the nascent RPL30 transcript.

Keywords

poylpyrimidine tract (Ppy tract), branch site (BS), ' splice site (3'ss), 5' splice site (5'ss), intron, L30, RPL30, U2 snRNP, U1 snRNP, transcription, regulation of splicing, spliceosome assembly, splicing, gene expression