In vivo detection of perinatal brain metabolite changes in a rabbit model of intrauterine growth restriction (IUGR)

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  • dc.contributor.author Simões, Rui Vasco
  • dc.contributor.author Muñoz-Moreno, Emma
  • dc.contributor.author Carbajo, Rodrigo J.
  • dc.contributor.author González-Tendero, Anna
  • dc.contributor.author Illa, Miriam
  • dc.contributor.author Sanz-Cortés, Magdalena
  • dc.contributor.author Pineda-Lucena, Antonio
  • dc.contributor.author Gratacós Solsona, Eduard
  • dc.date.accessioned 2025-12-02T06:48:05Z
  • dc.date.available 2025-12-02T06:48:05Z
  • dc.date.issued 2015
  • dc.description.abstract Background. Intrauterine growth restriction (IUGR) is a risk factor for abnormal neurodevelopment. We studied a rabbit model of IUGR by magnetic resonance imaging (MRI) and spectroscopy (MRS), to assess in vivo brain structural and metabolic consequences, and identify potential metabolic biomarkers for clinical translation. Methods. IUGR was induced in 3 pregnant rabbits at gestational day 25, by 40–50% uteroplacental vessel ligation in one horn; the contralateral horn was used as control. Fetuses were delivered at day 30 and weighted. A total of 6 controls and 5 IUGR pups underwent T2-w MRI and localized proton MRS within the first 8 hours of life, at 7T. Changes in brain tissue volumes and respective contributions to each MRS voxel were estimated by semi-automated registration of MRI images with a digital atlas of the rabbit brain. MRS data were used for: (i) absolute metabolite quantifications, using linear fitting; (ii) local temperature estimations, based on the water chemical shift; and (iii) classification, using spectral pattern analysis. Results. Lower birth weight was associated with (i) smaller brain sizes, (ii) slightly lower brain temperatures, and (iii) differential metabolite profile changes in specific regions of the brain parenchyma. Specifically, we found estimated lower levels of aspartate and N-acetylaspartate (NAA) in the cerebral cortex and hippocampus (suggesting neuronal impairment), and higher glycine levels in the striatum (possible marker of brain injury). Our results also suggest that the metabolic changes in cortical regions are more prevalent than those detected in hippocampus and striatum. Conclusions. IUGR was associated with brain metabolic changes in vivo, which correlate well with the neurostructural changes and neurodevelopment problems described in IUGR. Metabolic parameters could constitute non invasive biomarkers for the diagnosis and abnormal neurodevelopment of perinatal origin.
  • dc.description.sponsorship This work funded by grants from Obra Social “La Caixa” (Barcelona, Spain), Fundación Dexeus (Barcelona, Spain), AGAUR 2014 SGR (grant n° 928), and Ministerio de Ciencia e Innovación (SAF2011-28350). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Simões RV, Muñoz-Moreno E, Carbajo RJ, González-Tendero A, Illa M, Sanz-Cortés M, et al. In vivo detection of perinatal brain metabolite changes in a rabbit model of intrauterine growth restriction (IUGR). PLoS One. 2015 Jul 24;10(7):e0131310. DOI: 10.1371/journal.pone.0131310
  • dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0131310
  • dc.identifier.issn 1932-6203
  • dc.identifier.uri http://hdl.handle.net/10230/72086
  • dc.language.iso eng
  • dc.publisher Public Library of Science (PLoS)
  • dc.relation.ispartof PLoS One. 2015 Jul 24;10(7):e0131310
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2011-28350
  • dc.rights © 2015 Simões et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.other Metabòlits
  • dc.subject.other Conills
  • dc.subject.other Hipocamp
  • dc.title In vivo detection of perinatal brain metabolite changes in a rabbit model of intrauterine growth restriction (IUGR)
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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