Sam68 and circRNA biogenesis in early development

Antonelli, Maria Carla

Sam68 and circRNA biogenesis in early development

Document Type

Doctoral thesis
Published Version

Author

Antonelli, Maria Carla

Director

Tartaglia, Gian Gaetano
Bechara, Elias

Publication Date

2021-03-24

Pages

150 p.

Citation

Antonelli, M. Sam68 and circRNA biogenesis in early development. 2021. handle: http://hdl.handle.net/10803/671430

This citation was generated automatically.

Doctoral program

Programa de doctorat en Biomedicina

Abstract

During the last decades the non-coding portion of the genome became subject of intense research due to its contribution to the complexity of biological and pathological processes. CircRNAs are a class of non-coding RNAs able to influence gene expression during development and their biogenesis relies on the activity of several RBPs. Indeed, the Signal Transduction and Activation of RNA (STAR) family members Quaking and Sam68 have been shown to be involved in the biogenesis of circRNAs in specific biological contexts, but whether their contribution is extended to embryonic development is unknown. Our evidences suggest that Sam68 is involved in circRNA biogenesis during differentiation towards cardiac lineage, providing a new layer of regulation during embryonic development.
Durante las últimas décadas, la parte no codificante del genoma se ha convertido en objeto de intensa investigación debido a su contribución a la complejidad de los procesos biológicos y patológicos. Los CircRNAs son una clase de RNA no codificante capaces de influir en la expresión génica durante el desarrollo y su biogénesis se basa en la actividad de varias proteínas que ligan el RNA llamadas RNA-Binding Proteins (RBPs). Se ha demostrado que varias RBPs están implicadas en la biogénesis de los circRNAs, especialmente Quaking y Sam68, miembros de la familia STAR (Signal Transduction and Activation of RNA) pero se desconoce si su contribución se extiende al desarrollo embrionario. Mi proyecto de doctorado tiene como objetivo desenredar esta brecha definiendo el papel de Sam68 en la biogénesis de circRNAs durante la diferenciación de células madre embrionarias (mESCs).