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Profiling the secretome and extracellular proteome of the potato late blight pathogen phytophthora infestans

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dc.contributor.author Meijer, Harold J. G.
dc.contributor.author Mancuso, Francesco M.
dc.contributor.author Espadas, Guadalupe
dc.contributor.author Seidl, Michael F.
dc.contributor.author Chiva, Cristina
dc.contributor.author Govers, Francine
dc.contributor.author Sabidó Aguadé, Eduard, 1981-
dc.date.accessioned 2024-03-26T07:07:11Z
dc.date.available 2024-03-26T07:07:11Z
dc.date.issued 2014
dc.identifier.citation Meijer HJG, Mancuso FM, Espadas G, Seidl MF, Chiva C, Govers F, et al. Profiling the secretome and extracellular proteome of the potato late blight pathogen phytophthora infestans. Molecular & Cellular Proteomics. 2014 Aug;13(8):2101-13. DOI: 10.1074/mcp.M113.035873
dc.identifier.issn 1535-9476
dc.identifier.uri http://hdl.handle.net/10230/59555
dc.description.abstract Oomycetes are filamentous organisms that cause notorious diseases, several of which have a high economic impact. Well known is Phytophthora infestans, the causal agent of potato late blight. Previously, in silico analyses of the genome and transcriptome of P. infestans resulted in the annotation of a large number of genes encoding proteins with an N-terminal signal peptide. This set is collectively referred to as the secretome and comprises proteins involved in, for example, cell wall growth and modification, proteolytic processes, and the promotion of successful invasion of plant cells. So far, proteomic profiling in oomycetes was primarily focused on subcellular, intracellular or cell wall fractions; the extracellular proteome has not been studied systematically. Here we present the first comprehensive characterization of the in vivo secretome and extracellular proteome of P. infestans. We have used mass spectrometry to analyze P. infestans proteins present in seven different growth media with mycelial cultures and this resulted in the consistent identification of over two hundred proteins. Gene ontology classification pinpointed proteins involved in cell wall modifications, pathogenesis, defense responses, and proteolytic processes. Moreover, we found members of the RXLR and CRN effector families as well as several proteins lacking an obvious signal peptide. The latter were confirmed to be bona fide extracellular proteins and this suggests that, similar to other organisms, oomycetes exploit non-conventional secretion mechanisms to transfer certain proteins to the extracellular environment.
dc.description.sponsorship This work has been supported by the PRIME-XS project, grant agreement number 262067, funded by the European Union 7th Framework Programme Programme (FP7/2007–2013). We thank NWO-STW for the VIDI grant (Nr. 10281) to H.J.G.M. The CRG/UPF Proteomics Unit is part of the Spanish Proteomics Platform “Plataforma de Recursos Biomoleculares y Bioinformáticos” (ProteoRed, Instituto de Salud Carlos III), and it is co-funded by the European Regional Development Fund (ERDF) in the framework of the Operational Programme of Catalonia 2007–2013. Objective 2 of regional competitiveness and employment. We also acknowledge support of the Spanish Ministry of Economy and Competitiveness, through the ‘Centro de Excelencia Severo Ochoa 2013–2017‘ program (SEV-2012–0208).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher American Society for Biochemistry and Molecular Biology (ASBMB)
dc.relation.ispartof Molecular & Cellular Proteomics. 2014 Aug;13(8):2101-13
dc.rights This research was originally published in Journal Name. Author(s). Title. Journal Name. Year; Vol:pp-pp. © the American Society for Biochemistry and Molecular Biology.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.other Genomes
dc.subject.other Genètica
dc.subject.other Cèl·lules
dc.title Profiling the secretome and extracellular proteome of the potato late blight pathogen phytophthora infestans
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1074/mcp.M113.035873
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/262067
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SEV-2012-0208
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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