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Transition of human γ-tubulin ring complex into a closed conformation during microtubule nucleation

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dc.contributor.author Brito, Cláudia
dc.contributor.author Serna, Marina
dc.contributor.author Guerra, Pablo
dc.contributor.author Llorca, Óscar
dc.contributor.author Surrey, Thomas
dc.date.accessioned 2024-02-14T16:52:33Z
dc.date.available 2024-02-14T16:52:33Z
dc.date.issued 2024
dc.identifier.citation Brito C, Serna M, Guerra P, Llorca O, Surrey T. Transition of human γ-tubulin ring complex into a closed conformation during microtubule nucleation. Science. 2024 Feb 23;383(6685):870-6. DOI: 10.1126/science.adk6160
dc.identifier.issn 0036-8075
dc.identifier.uri http://hdl.handle.net/10230/59109
dc.description.abstract Microtubules are essential for intracellular organization and chromosome segregation. They are nucleated by the γ-tubulin ring complex (γTuRC). However, isolated vertebrate γTuRC adopts an open conformation that deviates from the microtubule structure, raising the question of the nucleation mechanism. Here we determine cryo-electron microscopy structures of human γTuRC bound to a nascent microtubule. Structural changes of the complex into a closed conformation ensure that γTuRC templates the 13-protofilament microtubules that exist in human cells. Closure is mediated by a latch that interacts with incorporating tubulin, making it part of the closing mechanism. Further rearrangements involve all γ-tubulin ring complex subunits and the removal of the actin-containing luminal bridge. Our proposed mechanism of microtubule nucleation by human γTuRC relies on large-scale structural changes that are likely the target of regulation in cells.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher American Association for the Advancement of Science (AAAS)
dc.relation.ispartof Science. 2024 Feb 23;383(6685):870-6
dc.rights This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science, DOI: 10.1126/science.adk6160.
dc.subject.other Microtúbuls
dc.subject.other Tubulines
dc.title Transition of human γ-tubulin ring complex into a closed conformation during microtubule nucleation
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1126/science.adk6160
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/951430
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2020-001049-S
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-108415GB-I00
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-114429RB-I00
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/acceptedVersion

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