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The cellular landscape of the endochondral bone during the transition to extrauterine life

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dc.contributor.author Díaz Rueda, Alejandro
dc.contributor.author Salvador-Martínez, Irepan
dc.contributor.author Sospedra-Arrufat, Ismael
dc.contributor.author Alcaina-Caro, Ana
dc.contributor.author Fernández-Miñán, Ana
dc.contributor.author Burgos-Ruiz, Ana M.
dc.contributor.author Cases, Ildefonso
dc.contributor.author Mohedano, Alberto
dc.contributor.author Tena, Juan J.
dc.contributor.author Heyn, Holger
dc.contributor.author López Ríos, Javier
dc.contributor.author Nusspaumer, Gretel
dc.date.accessioned 2024-02-13T07:19:07Z
dc.date.available 2024-02-13T07:19:07Z
dc.date.issued 2024
dc.identifier.citation Rueda AD, Salvador-Martínez I, Sospedra-Arrufat I, Alcaina-Caro A, Fernández-Miñán A, Burgos-Ruiz AM, Cases I, Mohedano A, Tena JJ, Heyn H, Lopez-Rios J, Nusspaumer G. The cellular landscape of the endochondral bone during the transition to extrauterine life. Immunol Cell Biol. 2024 Feb;102(2):131-48. DOI: 10.1111/imcb.12718
dc.identifier.issn 0818-9641
dc.identifier.uri http://hdl.handle.net/10230/59090
dc.description.abstract The cellular complexity of the endochondral bone underlies its essential and pleiotropic roles during organismal life. While the adult bone has received significant attention, we still lack a deep understanding of the perinatal bone cellulome. Here, we have profiled the full composition of the murine endochondral bone at the single-cell level during the transition from fetal to newborn life and in comparison with the adult tissue, with particular emphasis on the mesenchymal compartment. The perinatal bone contains different fibroblastic clusters with blastema-like characteristics in organizing and supporting skeletogenesis, angiogenesis and hematopoiesis. Our data also suggest dynamic inter- and intra-compartment interactions, as well as a bone marrow milieu that seems prone to anti-inflammation, which we hypothesize is necessary to ensure the proper program of lymphopoiesis and the establishment of central and peripheral tolerance in early life. Our study provides an integrative roadmap for the future design of genetic and cellular functional assays to validate cellular interactions and lineage relationships within the perinatal bone.
dc.description.sponsorship This work was supported by the Junta de Andalucía (PY20-00421 to JL-R) and the Spanish Ministerio de Ciencia e Innovación (María de Maeztu Institutional Grant CEX2020-001088-M to JL-R and JJT). We thank the rest of the members of the groups for scientific discussions and technical help. We are also grateful to A Franco, C Mateos, A López, P López and L Pérez for excellent mouse husbandry as well as the rest of the CABD Core services, in particular C Díaz (Flow Cytometry Facility). This manuscript was peer reviewed as a preprint by Review Commons prior to transfer to Immunology & Cell Biology.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartof Immunol Cell Biol. 2024 Feb;102(2):131-48
dc.rights © 2024 The Authors. Immunology & Cell Biology published by John Wiley & Sons Australia, Ltd on behalf of the Australian and New Zealand Society for Immunology, Inc. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.title The cellular landscape of the endochondral bone during the transition to extrauterine life
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1111/imcb.12718
dc.subject.keyword Birth
dc.subject.keyword Endochondral ossification
dc.subject.keyword Fibroblastmesenchymal stromal skeletal progenitors
dc.subject.keyword Mouse
dc.subject.keyword scRNA-seq
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2020-001088-M
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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