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A human in vitro neuronal model for studying homeostatic plasticity at the network level

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dc.contributor.author Yuan, Xiuming
dc.contributor.author Puvogel, Sofía
dc.contributor.author van Rhijn, Jon-Ruben
dc.contributor.author Ciptasari, Ummi
dc.contributor.author Esteve-Codina, Anna
dc.contributor.author Meijer, Mandy
dc.contributor.author Rouschop, Simon
dc.contributor.author van Hugte, Eline J. H.
dc.contributor.author Oudakker, Astrid
dc.contributor.author Schoenmaker, Chantal
dc.contributor.author Frega, Monica
dc.contributor.author Schubert, Dirk
dc.contributor.author Franke, Barbara
dc.contributor.author Nadif Kasri, Nael
dc.date.accessioned 2024-02-12T06:36:45Z
dc.date.available 2024-02-12T06:36:45Z
dc.date.issued 2023
dc.identifier.citation Yuan X, Puvogel S, van Rhijn JR, Ciptasari U, Esteve-Codina A, Meijer M, Rouschop S, van Hugte EJH, Oudakker A, Schoenmaker C, Frega M, Schubert D, Franke B, Nadif Kasri N. A human in vitro neuronal model for studying homeostatic plasticity at the network level. Stem Cell Reports. 2023 Nov 14;18(11):2222-39. DOI: 10.1016/j.stemcr.2023.09.011
dc.identifier.issn 2213-6711
dc.identifier.uri http://hdl.handle.net/10230/59060
dc.description.abstract Mechanisms that underlie homeostatic plasticity have been extensively investigated at single-cell levels in animal models, but are less well understood at the network level. Here, we used microelectrode arrays to characterize neuronal networks following induction of homeostatic plasticity in human induced pluripotent stem cell (hiPSC)-derived glutamatergic neurons co-cultured with rat astrocytes. Chronic suppression of neuronal activity through tetrodotoxin (TTX) elicited a time-dependent network re-arrangement. Increased expression of AMPA receptors and the elongation of axon initial segments were associated with increased network excitability following TTX treatment. Transcriptomic profiling of TTX-treated neurons revealed up-regulated genes related to extracellular matrix organization, while down-regulated genes related to cell communication; also astrocytic gene expression was found altered. Overall, our study shows that hiPSC-derived neuronal networks provide a reliable in vitro platform to measure and characterize homeostatic plasticity at network and single-cell levels; this platform can be extended to investigate altered homeostatic plasticity in brain disorders.
dc.description.sponsorship The work was supported by funding from the European Community’s Horizon 2020 Programme (H2020/2014–2020) under grant agreement no. 728018 (Eat2beNICE) (to B.F.); ERA-NET NEURON-102 SYNSCHIZ grant (NWO) 013-17-003 4538 (to D.S.); China Scholarship Council 201906100038 (to X.Y.); ISCIII /MINECO (PT17/0009/0019) and FEDER (to A.E.C.); and M.M. was supported by an internal grant from the Donders Centre for Medical Neurosciences of the Radboud University Medical Center.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof Stem Cell Reports. 2023 Nov 14;18(11):2222-39
dc.rights © 2023 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.title A human in vitro neuronal model for studying homeostatic plasticity at the network level
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.stemcr.2023.09.011
dc.subject.keyword hiPSC
dc.subject.keyword Homeostatic plasticity
dc.subject.keyword Human neuronal networks
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/728018
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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