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De novo transcriptome sequencing and gene co-expression reveal a genomic basis for drought sensitivity and evidence of a rapid local adaptation on Atlas cedar (Cedrus atlantica)

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dc.contributor.author Cobo-Simón, Irene
dc.contributor.author Gómez-Garrido, Jèssica
dc.contributor.author Esteve-Codina, Anna
dc.contributor.author Dabad, Marc
dc.contributor.author Alioto, Tyler
dc.contributor.author Maloof, Julin N.
dc.contributor.author Méndez-Cea, Belén
dc.contributor.author Seco, José Ignacio
dc.contributor.author Linares, Juan Carlos
dc.contributor.author Gallego, Francisco Javier
dc.date.accessioned 2023-06-21T06:34:44Z
dc.date.available 2023-06-21T06:34:44Z
dc.date.issued 2023
dc.identifier.citation Cobo-Simón I, Gómez-Garrido J, Esteve-Codina A, Dabad M, Alioto T, Maloof JN, Méndez-Cea B, Seco JI, Linares JC, Gallego FJ. De novo transcriptome sequencing and gene co-expression reveal a genomic basis for drought sensitivity and evidence of a rapid local adaptation on Atlas cedar (Cedrus atlantica). Front Plant Sci. 2023 Apr 19;14:1116863. DOI: 10.3389/fpls.2023.1116863
dc.identifier.issn 1664-462X
dc.identifier.uri http://hdl.handle.net/10230/57283
dc.description.abstract Introduction: Understanding the adaptive capacity to current climate change of drought-sensitive tree species is mandatory, given their limited prospect of migration and adaptation as long-lived, sessile organisms. Knowledge about the molecular and eco-physiological mechanisms that control drought resilience is thus key, since water shortage appears as one of the main abiotic factors threatening forests ecosystems. However, our current background is scarce, especially in conifers, due to their huge and complex genomes. Methods: Here we investigated the eco-physiological and transcriptomic basis of drought response of the climate change-threatened conifer Cedrus atlantica. We studied C. atlantica seedlings from two locations with contrasting drought conditions to investigate a local adaptation. Seedlings were subjected to experimental drought conditions, and were monitored at immediate (24 hours) and extended (20 days) times. In addition, post-drought recovery was investigated, depicting two contrasting responses in both locations (drought resilient and non-resilient). Single nucleotide polymorphisms (SNPs) were also studied to characterize the genomic basis of drought resilience and investigate a rapid local adaptation of C. atlantica. Results: De novo transcriptome assembly was performed for the first time in this species, providing differences in gene expression between the immediate and extended treatments, as well as among the post-drought recovery phenotypes. Weighted gene co-expression network analysis showed a regulation of stomatal closing and photosynthetic activity during the immediate drought, consistent with an isohydric dynamic. During the extended drought, growth and flavonoid biosynthesis inhibition mechanisms prevailed, probably to increase root-to-shoot ratio and to limit the energy-intensive biosynthesis of secondary metabolites. Drought sensitive individuals failed in metabolism and photosynthesis regulation under drought stress, and in limiting secondary metabolite production. Moreover, genomic differences (SNPs) were found between drought resilient and sensitive seedlings, and between the two studied locations, which were mostly related to transposable elements. Discussion: This work provides novel insights into the transcriptomic basis of drought response of C. atlantica, a set of candidate genes mechanistically involved in its drought sensitivity and evidence of a rapid local adaptation. Our results may help guide conservation programs for this threatened conifer, contribute to advance drought-resilience research and shed light on trees' adaptive potential to current climate change.
dc.description.sponsorship IC-S was supported by a Predoctoral Grant BES-2014-070379 and a Predoctoral Mobility Grant EEBB-I-18-12943, Spanish Ministry of Economy. This study was supported by the projects CGL2013-48843-C2-2-R, Spanish Ministry of Economy; TED2021-129770B-C22 and PID2021-123675OB-C44, Spanish Ministry of Science and Innovation.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Frontiers
dc.relation.ispartof Front Plant Sci. 2023 Apr 19;14:1116863
dc.rights © 2023 Cobo-Simón, Gómez-Garrido, Esteve-Codina, Dabad, Alioto, Maloof, Méndez-Cea, Seco, Linares and Gallego. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title De novo transcriptome sequencing and gene co-expression reveal a genomic basis for drought sensitivity and evidence of a rapid local adaptation on Atlas cedar (Cedrus atlantica)
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.3389/fpls.2023.1116863
dc.subject.keyword Atlas cedar
dc.subject.keyword RNA-Seq
dc.subject.keyword Adaptive capacity
dc.subject.keyword Climate change
dc.subject.keyword Conifers
dc.subject.keyword Drought sensitiveness
dc.subject.keyword Eco-physiology
dc.subject.keyword Phenotypic diversity
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/CGL2013-48843-C2-2-R
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/TED2021-129770B-C22
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2021-123675OB-C44
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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