Browsing Recerca: articles, congressos, llibres by Subject "DNA synthesis"

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Budding yeast complete DNA synthesis after chromosome segregation begins

Ivanova, Tsvetomira Georgieva, 1978-; Maier, Michael, 1983-; Missarova, Alsu, 1990-; Ziegler-Birling, Céline; Dam, Monica; Gomar-Alba, Mercè; Carey, Lucas, 1980-; Mendoza, Manuel (Mendoza Palomares) (Nature Research, 2020)

To faithfully transmit genetic information, cells must replicate their entire genome before division. This is thought to be ensured by the temporal separation of replication and chromosome segregation. Here we show that ...
SUNi mutagenesis: Scalable and uniform nicking for efficient generation of variant libraries

Mighell, Taylor L.; Toledano, Ignasi; Lehner, Ben, 1978- (Public Library of Science (PLoS), 2023)

Multiplexed assays of variant effects (MAVEs) have made possible the functional assessment of all possible mutations to genes and regulatory sequences. A core pillar of the approach is generation of variant libraries, but ...

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