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Cross-linker design determines microtubule network organization by opposing motors

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dc.contributor.author Henkin, Gil
dc.contributor.author Chew, Wei-Xiang
dc.contributor.author Nédélec, François
dc.contributor.author Surrey, Thomas
dc.date.accessioned 2022-11-08T07:12:55Z
dc.date.available 2022-11-08T07:12:55Z
dc.date.issued 2022
dc.identifier.citation Henkin G, Chew WX, Nédélec F, Surrey T. Cross-linker design determines microtubule network organization by opposing motors. Proc Natl Acad Sci U S A. 2022 Aug 16;119(33):e2206398119. DOI: 10.1073/pnas.2206398119
dc.identifier.issn 0027-8424
dc.identifier.uri http://hdl.handle.net/10230/54744
dc.description.abstract During cell division, cross-linking motors determine the architecture of the spindle, a dynamic microtubule network that segregates the chromosomes in eukaryotes. It is unclear how motors with opposite directionality coordinate to drive both contractile and extensile behaviors in the spindle. Particularly, the impact of different cross-linker designs on network self-organization is not understood, limiting our understanding of self-organizing structures in cells but also our ability to engineer new active materials. Here, we use experiment and theory to examine active microtubule networks driven by mixtures of motors with opposite directionality and different cross-linker design. We find that although the kinesin-14 HSET causes network contraction when dominant, it can also assist the opposing kinesin-5 KIF11 to generate extensile networks. This bifunctionality results from HSET's asymmetric design, distinct from symmetric KIF11. These findings expand the set of rules underlying patterning of active microtubule assemblies and allow a better understanding of motor cooperation in the spindle.
dc.description.sponsorship This work was supported by the Spanish Ministry of Economy, Industry and Competitiveness to the CRG-EMBL partnership, the Centro de Excelencia Severo Ochoa and the CERCA Programme of the Generalitat de Catalunya, and the Francis Crick Institute, which receives its core funding from Cancer Research UK (FC001163), the UK Medical Research Council (FC001163), and the Wellcome Trust (FC001163). W.-X.C. is supported by a Human Frontier Science Program fellowship (HFSP LT000682/2020-C). F.N. is supported by the Gatsby Charitable Foundation (Grant PTAG-024) and the European Research Council (ERC Synergy Grant, Project 951430). T.S. acknowledges support from the European Research Council (ERC Advanced Grant, Project 323042, ERC Synergy Grant, Project 951430).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher National Academy of Sciences
dc.relation.ispartof Proc Natl Acad Sci U S A. 2022 Aug 16;119(33):e2206398119
dc.rights © 2022 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.title Cross-linker design determines microtubule network organization by opposing motors
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1073/pnas.2206398119
dc.subject.keyword Active matter
dc.subject.keyword Microtubules
dc.subject.keyword Motor proteins
dc.subject.keyword Self-organization
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/951430
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/323042
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/951430
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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