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Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters

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dc.contributor.author Robles-Rebollo, Irene
dc.contributor.author Cuartero, Sergi
dc.contributor.author Canellas-Socias, Adria
dc.contributor.author Wells, Sarah
dc.contributor.author Karimi, Mohammad M.
dc.contributor.author Mereu, Elisabetta
dc.contributor.author Chivu, Alexandra G.
dc.contributor.author Heyn, Holger
dc.contributor.author Whilding, Chad
dc.contributor.author Dormann, Dirk
dc.contributor.author Marguerat, Samuel
dc.contributor.author Rioja, Inmaculada
dc.contributor.author Prinjha, Rab K.
dc.contributor.author Stumpf, Michael P.H.
dc.contributor.author Fisher, Amanda G.
dc.contributor.author Merkenschlager, Matthias
dc.date.accessioned 2022-11-04T07:45:45Z
dc.date.available 2022-11-04T07:45:45Z
dc.date.issued 2022
dc.identifier.citation Robles-Rebollo I, Cuartero S, Canellas-Socias A, Wells S, Karimi MM, Mereu E, Chivu AG, Heyn H, Whilding C, Dormann D, Marguerat S, Rioja I, Prinjha RK, Stumpf MPH, Fisher AG, Merkenschlager M. Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters. Nat Commun. 2022 Jul 27;13(1):4342. DOI: 10.1038/s41467-022-31192-9
dc.identifier.issn 2041-1723
dc.identifier.uri http://hdl.handle.net/10230/54693
dc.description.abstract Innate immune responses rely on inducible gene expression programmes which, in contrast to steady-state transcription, are highly dependent on cohesin. Here we address transcriptional parameters underlying this cohesin-dependence by single-molecule RNA-FISH and single-cell RNA-sequencing. We show that inducible innate immune genes are regulated predominantly by an increase in the probability of active transcription, and that probabilities of enhancer and promoter transcription are coordinated. Cohesin has no major impact on the fraction of transcribed inducible enhancers, or the number of mature mRNAs produced per transcribing cell. Cohesin is, however, required for coupling the probabilities of enhancer and promoter transcription. Enhancer-promoter coupling may not be explained by spatial proximity alone, and at the model locus Il12b can be disrupted by selective inhibition of the cohesinopathy-associated BET bromodomain BD2. Our data identify discrete steps in enhancer-mediated inducible gene expression that differ in cohesin-dependence, and suggest that cohesin and BD2 may act on shared pathways.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Nature Research
dc.relation.ispartof Nat Commun. 2022 Jul 27;13(1):4342
dc.rights © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1038/s41467-022-31192-9
dc.subject.keyword Nuclear organization
dc.subject.keyword Transcription
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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