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A role for the fusogen eff-1 in epidermal stem cell number robustness in Caenorhabditis elegans

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dc.contributor.author Koneru, Shena L.
dc.contributor.author Quah, Fu Xiang
dc.contributor.author Ghose, Ritobrata
dc.contributor.author Hintze, Mark
dc.contributor.author Gritti, Nicola
dc.contributor.author van Zon, Jeroen Sebastiaan
dc.contributor.author Barkoulas, Michalis
dc.date.accessioned 2022-06-13T08:56:01Z
dc.date.available 2022-06-13T08:56:01Z
dc.date.issued 2021
dc.identifier.citation Koneru SL, Quah FX, Ghose R, Hintze M, Gritti N, van Zon JS et al. A role for the fusogen eff-1 in epidermal stem cell number robustness in Caenorhabditis elegans. Sci Rep. 2021 May 7;11(1):9787. DOI:10.1038/s41598-021-88500-4
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10230/53473
dc.description.abstract Developmental patterning in Caenorhabditis elegans is known to proceed in a highly stereotypical manner, which raises the question of how developmental robustness is achieved despite the inevitable stochastic noise. We focus here on a population of epidermal cells, the seam cells, which show stem cell-like behaviour and divide symmetrically and asymmetrically over post-embryonic development to generate epidermal and neuronal tissues. We have conducted a mutagenesis screen to identify mutants that introduce phenotypic variability in the normally invariant seam cell population. We report here that a null mutation in the fusogen eff-1 increases seam cell number variability. Using time-lapse microscopy and single molecule fluorescence hybridisation, we find that seam cell division and differentiation patterns are mostly unperturbed in eff-1 mutants, indicating that cell fusion is uncoupled from the cell differentiation programme. Nevertheless, seam cell losses due to the inappropriate differentiation of both daughter cells following division, as well as seam cell gains through symmetric divisions towards the seam cell fate were observed at low frequency. We show that these stochastic errors likely arise through accumulation of defects interrupting the continuity of the seam and changing seam cell shape, highlighting the role of tissue homeostasis in suppressing phenotypic variability during development.
dc.description.sponsorship Funding: Some C. elegans strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). This work was funded by the European Research Council (ROBUSTNET-639485)
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Nature Research
dc.rights © Sneha L. Koneru et al 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject.other Caenorhabditis elegans
dc.subject.other Genètica
dc.subject.other Cèl·lules
dc.title A role for the fusogen eff-1 in epidermal stem cell number robustness in Caenorhabditis elegans
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1038/s41598-021-88500-4
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/639485
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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