dc.contributor.author |
Bauer, Moritz, 1987- |
dc.contributor.author |
Vidal, Enrique |
dc.contributor.author |
Zorita, Eduard |
dc.contributor.author |
Üresin, Nil |
dc.contributor.author |
Pinter, Stefan F. |
dc.contributor.author |
Filion, Guillaume |
dc.contributor.author |
Payer, Bernhard |
dc.date.accessioned |
2021-11-24T07:40:34Z |
dc.date.available |
2021-11-24T07:40:34Z |
dc.date.issued |
2021 |
dc.identifier.citation |
Bauer M, Vidal E, Zorita E, Üresin N, Pinter SF, Filion GJ, Payer B. Chromosome compartments on the inactive X guide TAD formation independently of transcription during X-reactivation. Nat Commun. 2021;12(1):3499. DOI: 10.1038/s41467-021-23610-1 |
dc.identifier.issn |
2041-1723 |
dc.identifier.uri |
http://hdl.handle.net/10230/49045 |
dc.description.abstract |
A hallmark of chromosome organization is the partition into transcriptionally active A and repressed B compartments, and into topologically associating domains (TADs). Both structures were regarded to be absent from the inactive mouse X chromosome, but to be re-established with transcriptional reactivation and chromatin opening during X-reactivation. Here, we combine a tailor-made mouse iPSC reprogramming system and high-resolution Hi-C to produce a time course combining gene reactivation, chromatin opening and chromosome topology during X-reactivation. Contrary to previous observations, we observe A/B-like compartments on the inactive X harbouring multiple subcompartments. While partial X-reactivation initiates within a compartment rich in X-inactivation escapees, it then occurs rapidly along the chromosome, concomitant with downregulation of Xist. Importantly, we find that TAD formation precedes transcription and initiates from Xist-poor compartments. Here, we show that TAD formation and transcriptional reactivation are causally independent during X-reactivation while establishing Xist as a common denominator. |
dc.description.sponsorship |
This work was supported by the European Research Council under the 7th Framework Programme FP7/2007-2013 (ERC Synergy Grant 4D-Genome, grant agreement 609989 to G.J.F.), by the Spanish Ministry of Science, Innovation and Universities (BFU2014-55275-P, BFU2017-88407-P to B.P. and PGC2018-099807-B-I00 to G.J.F.), the Agencia Estatal de Investigación (AEI) (EUR2019-103817 to B.P.), the AXA Research Fund (to B.P.) and the Agencia de Gestio d’Ajuts Universitaris i de Recerca (AGAUR, 2017 SGR 346 to B.P.) and by the NIH grant R35GM124926 to S.F.P. We would like to thank the Spanish Ministry of Economy, Industry and Competitiveness (MEIC) to the EMBL partnership and to the “Centro de Excelencia Severo Ochoa”. We also acknowledge support of the CERCA Programme of the Generalitat de Catalunya. M.B. was supported by a La Caixa International PhD Fellowship. |
dc.format.mimetype |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Nature Research |
dc.relation.ispartof |
Nat Commun. 2021;12(1):3499 |
dc.rights |
© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
dc.rights.uri |
http://creativecommons.org/licenses/by/4.0/ |
dc.title |
Chromosome compartments on the inactive X guide TAD formation independently of transcription during X-reactivation |
dc.type |
info:eu-repo/semantics/article |
dc.identifier.doi |
http://dx.doi.org/10.1038/s41467-021-23610-1 |
dc.subject.keyword |
Chromatin structure |
dc.subject.keyword |
Dosage compensation |
dc.subject.keyword |
Epigenetics |
dc.subject.keyword |
Epigenomics |
dc.subject.keyword |
Reprogramming |
dc.relation.projectID |
info:eu-repo/grantAgreement/EC/FP7/609989 |
dc.relation.projectID |
info:eu-repo/grantAgreement/ES/1PE/BFU2014-55275-P |
dc.relation.projectID |
info:eu-repo/grantAgreement/ES/2PE/BFU2017-88407-P |
dc.relation.projectID |
info:eu-repo/grantAgreement/ES/2PE/PGC2018-099807-B-I00 |
dc.rights.accessRights |
info:eu-repo/semantics/openAccess |
dc.type.version |
info:eu-repo/semantics/publishedVersion |