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Diagnostic value of genotypic analysis in primary cutaneous lymphomas using standardized BIOMED-2 polymerase chain reaction protocols: experience in daily clinical practice

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dc.contributor.author López-Aventín, Daniel
dc.contributor.author Gallardo Hernández, Fernando
dc.contributor.author Colomo Saperas, Luis Alberto
dc.contributor.author Moragón, Ester
dc.contributor.author Vela Ortíz, María del Carmen
dc.contributor.author Duran Jordà, Xavier, 1974-
dc.contributor.author Bellosillo Paricio, Beatriz
dc.contributor.author Pujol Vallverdú, Ramon Maria
dc.date.accessioned 2021-08-26T06:54:29Z
dc.date.available 2021-08-26T06:54:29Z
dc.date.issued 2021
dc.identifier.citation López Aventín D, Gallardo F, Colomo L, Moragón E, Vela MC, Duran Jordà X, Bellosillo B, Pujol RM. Diagnostic value of genotypic analysis in primary cutaneous lymphomas using standardized BIOMED-2 polymerase chain reaction protocols: experience in daily clinical practice. Acta Derm Venereol. 2021;101(5):adv00460. DOI: 10.2340/00015555-3828
dc.identifier.issn 0001-5555
dc.identifier.uri http://hdl.handle.net/10230/48332
dc.description.abstract BIOMED-2 Concerted Action BMH4-CT98-3936 (BIOMED-2) PCR protocols are an important diagnostic tool in the evaluation of cutaneous lymphomas. The aim of this study was to assess the diagnostic value of the genotyping results obtained by these techniques in daily clinical practice. A total of 360 paraffin-embedded skin samples were retrospectively reviewed from 114 cutaneous T-cell lymphomas and 35 cutaneous B-cell lym-phomas. A total of 249 biopsies from 180 patients with benign lymphoid infiltrates served as controls. T-cell receptor and immunoglobulin gene rearrangements were assessed using the BIOMED-2 method. A combined T-cell receptor gamma and beta assay approach reliably distinguished cutaneous T-cell lymphomas from benign skin T-cell infiltrates (sensitivity 89.4%; specificity 81.5%). Analysis of complete immunoglobulin heavy chain rearrangements also differentiated cutaneous B-cell lymphomas from benign B-cell infiltrates (sensitivity 85.7%; specificity 82.4%). In conclusion, the full BIOMED-2 protocol is a useful aid combined with clinical, histological and immunophenotypical findings for assessment of lymphoid clonality in skin lymphoid proliferations.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Society for Publication of Acta Dermato-Venereologica
dc.relation.ispartof Acta Derm Venereol. 2021;101(5):adv00460
dc.rights This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/)
dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
dc.title Diagnostic value of genotypic analysis in primary cutaneous lymphomas using standardized BIOMED-2 polymerase chain reaction protocols: experience in daily clinical practice
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.2340/00015555-3828
dc.subject.keyword T-cell receptor genes
dc.subject.keyword Clonality
dc.subject.keyword Cutaneous B-cell lymphoma
dc.subject.keyword Cutaneous T-cell lymphoma
dc.subject.keyword Immunoglobulin genes
dc.subject.keyword Multiplex polymerase chain reaction
dc.subject.keyword BIOMED-2
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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