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Implementation of targeted proteomics methods for the characterization of peptides and proteins in liquid biopsies

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dc.contributor.author Vila Escutia, Laia
dc.date.accessioned 2020-12-21T11:36:13Z
dc.date.available 2020-12-21T11:36:13Z
dc.date.issued 2020
dc.identifier.uri http://hdl.handle.net/10230/46104
dc.description Treball de fi de grau en Biologia Humana
dc.description Supervisor: Eduard Sabidó Aguadé (CRG)
dc.description.abstract Targeted proteomics methods emerged to detect and quantify well-defined sets of peptides with a high degree of specificity and sensitivity. Internal standard triggered-PRM (IS-PRM) was designed to increase the number of peptides monitored in one analysis without affecting the quality of the data obtained by using stable isotopically labeled (SIL) peptides as internal standards. We aimed to investigate the technical reproducibility of a type of IS-PRM method called SureQuant and to quantify the added SIL peptides and their corresponding endogenous forms. Three aliquots of commercially available human serum samples were digested with trypsin and a set of 802 isotopically-labelled standard (SIL) peptides were spiked-in to later be analyzed by LC-MS using SureQuant acquisition method. Instrument variability showed a coefficient of variation (CV) of 9.3%, whereas samplepreparation variability increases up to 34.7% and 39.1%. The technique enabled to detect, on average, 772 SIL peptides and 738 endogenous forms. Data obtained shows very good instrument reproducibility, whereas sample preparation procedure presents greater variability. Thus, this study proves the need for an improvement to achieve reproducibility and obtain reliable quantitative results.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.rights © Tots els drets reservats
dc.subject.other Proteòmica
dc.subject.other Pèptids
dc.subject.other Proteïnes
dc.subject.other Biòpsia
dc.title Implementation of targeted proteomics methods for the characterization of peptides and proteins in liquid biopsies
dc.type info:eu-repo/semantics/bachelorThesis
dc.rights.accessRights info:eu-repo/semantics/openAccess

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