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Phenotypic variability in a coinfection with three independent Candida parapsilosis lineages

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dc.contributor.author Gómez-Molero, Emilia
dc.contributor.author Willis, Jesse R.
dc.contributor.author Dudakova, Anna
dc.contributor.author Carreté Muñoz, Laia, 1990-
dc.contributor.author Weig, Michael
dc.contributor.author Groß, Uwe
dc.contributor.author Gácser, Attila
dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-
dc.contributor.author Bader, Olivier
dc.date.accessioned 2020-11-10T07:05:47Z
dc.date.available 2020-11-10T07:05:47Z
dc.date.issued 2020
dc.identifier.citation Gómez-Molero E, Willis JR, Dudakova A, Carreté L, Weig M, Groß U, Gácser A, Gabaldón T, Bader O. Phenotypic variability in a coinfection with three independent Candida parapsilosis lineages. Front Microbiol. 2020; 11:1994. DOI: 10.3389/fmicb.2020.01994
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/10230/45695
dc.description.abstract The human pathogenic yeast Candida parapsilosis has gained significant importance over the past decades as one of the principal causes of fungal bloodstream infections. Isolates of C. parapsilosis are known to be able to switch between several different colony morphologies in vitro, which are correlated with different cell shapes, altered cell surface properties, and thus different capacities to form biofilms on indwelling medical devices. In a set of six clinical specimens from a single surgery patient yielding stable smooth- as well as crepe-morphology isolates, we investigated the differences between five of them on a phenotypic and genomic level. In contrast to the initial assumption that they were switched forms of a clonal strain, karyotyping and genome sequencing showed that the patient was colonized by at least three distinct linages. Statistical analysis placed these groups distantly across the population of C. parapsilosis. Interestingly the single blood culture isolate was of smooth morphology and matched with an isolate from the patient's nose of similar morphology. Strong variation between the isolates was seen in adhesin-encoding genes, where repeat regions showed significant variation in length and repeat-numbers, most strikingly in HWP1 of the smooth isolates. Although no differences in drug susceptibility were evident, the high phylogenetic distance separating the individual strains highlights the need for testing of multiple colonies in routine practice. The absence of biofilm formation in the blood stream isolate indicates a lack of respective adhesins in the cell wall, in turn pointing toward lack of adhesion as a positively contributing factor for dissemination.
dc.description.sponsorship This study was funded through the FP7-PEOPLE-2013-ITN—Marie-Curie Action: “Initial Training Networks”: Molecular Mechanisms of Human Fungal Pathogen Host Interaction, ImResFun, Project ID: 606786, to AG, TG, OB, and UG.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Frontiers
dc.relation.ispartof Front Microbiol. 2020; 11:1994
dc.rights © 2020 Gómez-Molero, Willis, Dudakova, Carreté, Weig, Groß, Gácser, Gabaldón and Bader. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Phenotypic variability in a coinfection with three independent Candida parapsilosis lineages
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.3389/fmicb.2020.01994
dc.subject.keyword Candida parapsilosis
dc.subject.keyword Adhesin genes
dc.subject.keyword Coinfection
dc.subject.keyword Genome sequencing
dc.subject.keyword Phenotypic variation
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/606786
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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