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Maternal spindle transfer overcomes embryo developmental arrest caused by ooplasmic defects in mice

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dc.contributor.author Costa Borges, Nuno Luis
dc.contributor.author Spath, Katharina
dc.contributor.author Miguel Escalada, Irene
dc.contributor.author Mestres, Enric
dc.contributor.author Balmaseda, Rosa
dc.contributor.author Serafín, Anna
dc.contributor.author Garcia Jiménez, Maria
dc.contributor.author Vanrell, Ivette
dc.contributor.author González, Jesús
dc.contributor.author Rink, Klaus
dc.contributor.author Wells, Dagan
dc.contributor.author Calderón, Gloria
dc.date.accessioned 2020-05-14T06:49:13Z
dc.date.available 2020-05-14T06:49:13Z
dc.date.issued 2020
dc.identifier.citation Costa-Borges N, Spath K, Miguel-Escalada I, Mestres E, Balmaseda R, Serafín A, Garcia-Jiménez M, Vanrell I, González J, Rink K, Wells D, Calderón G. Maternal spindle transfer overcomes embryo developmental arrest caused by ooplasmic defects in mice. Elife. 2020 Apr 29;9:e48591. DOI: 10.7554/eLife.48591
dc.identifier.issn 2050-084X
dc.identifier.uri http://hdl.handle.net/10230/44541
dc.description.abstract The developmental potential of early embryos is mainly dictated by the quality of the oocyte. Here, we explore the utility of the maternal spindle transfer (MST) technique as a reproductive approach to enhance oocyte developmental competence. Our proof-of-concept experiments show that replacement of the entire cytoplasm of oocytes from a sensitive mouse strain overcomes massive embryo developmental arrest characteristic of non-manipulated oocytes. Genetic analysis confirmed minimal carryover of mtDNA following MST. Resulting mice showed low heteroplasmy levels in multiple organs at adult age, normal histology and fertility. Mice were followed for 5 generations (F5), revealing that heteroplasmy was reduced in F2 mice and was undetectable in the subsequent generations. This pre-clinical model demonstrates the high efficiency and potential of the MST technique, not only to prevent the transmission of mtDNA mutations, but also as a new potential treatment for patients with certain forms of infertility refractory to current clinical strategies.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher eLife
dc.relation.ispartof Elife. 2020 Apr 29;9:e48591
dc.rights © 2020, Costa-Borges et al. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Maternal spindle transfer overcomes embryo developmental arrest caused by ooplasmic defects in mice
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.7554/eLife.48591
dc.subject.keyword Cell biology
dc.subject.keyword Developmental biology
dc.subject.keyword Mouse
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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