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CSDE1 controls gene expression through the miRNA-mediated decay machinery

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dc.contributor.author Kakumani, Pavan Kumar
dc.contributor.author Harvey, Louis-Mathieu
dc.contributor.author Houle, François
dc.contributor.author Guitart, Tanit
dc.contributor.author Gebauer, Fátima
dc.contributor.author Simard, Martin J.
dc.date.accessioned 2020-05-12T07:09:55Z
dc.date.available 2020-05-12T07:09:55Z
dc.date.issued 2020
dc.identifier.citation Kakumani PK, Harvey LM, Houle F, Guitart T, Gebauer F, Simard MJ. CSDE1 controls gene expression through the miRNA-mediated decay machinery. Life Sci Alliance. 2020; 3(4). pii: e201900632. DOI: 10.26508/lsa.201900632
dc.identifier.issn 2575-1077
dc.identifier.uri http://hdl.handle.net/10230/44489
dc.description.abstract In animals, miRNAs are the most prevalent small non-coding RNA molecules controlling posttranscriptional gene regulation. The Argonaute proteins (AGO) mediate miRNA-guided gene silencing by recruiting multiple factors involved in translational repression, deadenylation, and decapping. Here, we report that CSDE1, an RNA-binding protein linked to stem cell maintenance and metastasis in cancer, interacts with AGO2 within miRNA-induced silencing complex and mediates gene silencing through its N-terminal domains. We show that CSDE1 interacts with LSM14A, a constituent of P-body assembly and further associates to the DCP1-DCP2 decapping complex, suggesting that CSDE1 could promote the decay of miRNA-induced silencing complex-targeted mRNAs. Together, our findings uncover a hitherto unknown mechanism used by CSDE1 in the control of gene expression mediated by the miRNA pathway.
dc.description.sponsorship We also thank all members of our laboratory for helpful discussions and the mass spectrometry facility from the Centre Hospitalier Universitaire de Québec-Université Laval Research Center. Funding: The Canadian Institutes of Health Research supported this work (MJ Simard). PK Kakumani and LM Harvey are recipients of scholarship from the Fonds de Recherche du Québec-Santé (FRQ-S). MJ Simard is a Research Chair from FRQ-S. F Gebauer was supported by grants from the Spanish Ministry of Economy and Competitiveness (BFU2015-68741), the Catalan Government (2017 SGR 799), Marató de TV3 (TV′13-20131430), and the Centre of Excellence Severo Ochoa.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Life Science Alliance
dc.relation.ispartof Life Sci Alliance. 2020; 3(4). pii: e201900632
dc.rights © 2020 Kakumani et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.title CSDE1 controls gene expression through the miRNA-mediated decay machinery
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.26508/lsa.201900632
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68741
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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