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Recurrent somatic mutations reveal new insights into consequences of mutagenic processes in cancer

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dc.contributor.author Stobbe, Miranda D.
dc.contributor.author Thun, Gian Andri
dc.contributor.author Diéguez Docampo, Andrea
dc.contributor.author Oliva, Meritxell
dc.contributor.author Whalley, Justin P.
dc.contributor.author Raineri, Emanuele
dc.contributor.author Gut, Ivo Glynne
dc.date.accessioned 2020-04-07T08:47:49Z
dc.date.available 2020-04-07T08:47:49Z
dc.date.issued 2019
dc.identifier.citation Stobbe MD, Thun GA, Diéguez-Docampo A, Oliva M, Whalley JP, Raineri E, Gut IG. Recurrent somatic mutations reveal new insights into consequences of mutagenic processes in cancer. PLoS Comput Biol. 2019; 15(11):e1007496. DOI: 10.1371/journal.pcbi.1007496
dc.identifier.issn 1553-734X
dc.identifier.uri http://hdl.handle.net/10230/44176
dc.description.abstract The sheer size of the human genome makes it improbable that identical somatic mutations at the exact same position are observed in multiple tumours solely by chance. The scarcity of cancer driver mutations also precludes positive selection as the sole explanation. Therefore, recurrent mutations may be highly informative of characteristics of mutational processes. To explore the potential, we use recurrence as a starting point to cluster >2,500 whole genomes of a pan-cancer cohort. We describe each genome with 13 recurrence-based and 29 general mutational features. Using principal component analysis we reduce the dimensionality and create independent features. We apply hierarchical clustering to the first 18 principal components followed by k-means clustering. We show that the resulting 16 clusters capture clinically relevant cancer phenotypes. High levels of recurrent substitutions separate the clusters that we link to UV-light exposure and deregulated activity of POLE from the one representing defective mismatch repair, which shows high levels of recurrent insertions/deletions. Recurrence of both mutation types characterizes cancer genomes with somatic hypermutation of immunoglobulin genes and the cluster of genomes exposed to gastric acid. Low levels of recurrence are observed for the cluster where tobacco-smoke exposure induces mutagenesis and the one linked to increased activity of cytidine deaminases. Notably, the majority of substitutions are recurrent in a single tumour type, while recurrent insertions/deletions point to shared processes between tumour types. Recurrence also reveals susceptible sequence motifs, including TT[C>A]TTT and AAC[T>G]T for the POLE and 'gastric-acid exposure' clusters, respectively. Moreover, we refine knowledge of mutagenesis, including increased C/G deletion levels in general for lung tumours and specifically in midsize homopolymer sequence contexts for microsatellite instable tumours. Our findings are an important step towards the development of a generic cancer diagnostic test for clinical practice based on whole-genome sequencing that could replace multiple diagnostics currently in use.
dc.description.sponsorship We acknowledge the support of the Spanish Ministry of Economy, Industry and Competitiveness (MEIC) through the Instituto de Salud Carlos III and the 2014-2020 Smart Growth Operating Program, to the EMBL partnership and co-financing with the European Regional Development Fund (MINECO/FEDER, BIO2015-71792-P - awarded to IGG). We also acknowledge the support of the Centro de Excelencia Severo Ochoa, and the Generalitat de Catalunya through the Departament de Salut, Departament d’Empresa i Coneixement and the CERCA Programme. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PLoS Comput Biol. 2019; 15(11):e1007496
dc.rights © 2019 Stobbe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Recurrent somatic mutations reveal new insights into consequences of mutagenic processes in cancer
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1371/journal.pcbi.1007496
dc.subject.keyword Sequence motif analysis
dc.subject.keyword Cancer genomics
dc.subject.keyword Immunoglobulin genes
dc.subject.keyword Insertion mutation
dc.subject.keyword Somatic mutation
dc.subject.keyword Substitution mutation
dc.subject.keyword Hierarchical clustering
dc.subject.keyword Principal component analysis
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BIO2015-71792-P
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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