Welcome to the UPF Digital Repository

Microtubule nucleation during central spindle assembly requires NEDD1 phosphorylation on serine 405 by Aurora A

Show simple item record

dc.contributor.author Courthéoux, Thibault
dc.contributor.author Reboutier, David
dc.contributor.author Vazeille, Thibaut
dc.contributor.author Cremet, Jean-Yves
dc.contributor.author Benaud, Christelle
dc.contributor.author Vernos, Isabelle, 1959-
dc.contributor.author Prigent, Claude
dc.date.accessioned 2020-03-30T11:31:00Z
dc.date.issued 2019
dc.identifier.citation Courthéoux T, Reboutier D, Vazeille T, Cremet JY, Benaud C, Vernos I, Prigent C. Microtubule nucleation during central spindle assembly requires NEDD1 phosphorylation on serine 405 by Aurora A. J Cell Sci. 2019; 132(10). pii: jcs231118. DOI: 10.1242/jcs.231118
dc.identifier.issn 0021-9533
dc.identifier.uri http://hdl.handle.net/10230/44114
dc.description.abstract During mitosis, the cell sequentially constructs two microtubule-based spindles to ensure faithful segregation of chromosomes. A bipolar spindle first pulls apart the sister chromatids, then a central spindle further separates them away. Although the assembly of the first spindle is well described, the assembly of the second remains poorly understood. We report here that the inhibition of Aurora A leads to an absence of the central spindle resulting from a lack of nucleation of microtubules in the midzone. In the absence of Aurora A, the HURP (also known as DLGAP5) and NEDD1 proteins that are involved in nucleation of microtubules fail to concentrate in the midzone. HURP is an effector of RanGTP, whereas NEDD1 serves as an anchor for the γ-tubulin ring complex (γTURC). Interestingly, Aurora A phosphorylates HURP and NEDD1 during assembly of the initial bipolar spindle. We show here that the expression of a NEDD1 isoform mimicking phosphorylation by Aurora A is sufficient to restore microtubule nucleation in the midzone under conditions of Aurora A inhibition. These results reveal a new control mechanism of microtubule nucleation by Aurora A during assembly of the central spindle.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Company of Biologists
dc.relation.ispartof J Cell Sci. 2019; 132(10). pii: jcs231118
dc.rights © Company of Biologists http://dx.doi.org/10.1242/jcs.231118
dc.title Microtubule nucleation during central spindle assembly requires NEDD1 phosphorylation on serine 405 by Aurora A
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1242/jcs.231118
dc.subject.keyword Aurora A
dc.subject.keyword Central spindle
dc.subject.keyword Cytokinesis
dc.subject.keyword Microtubule
dc.subject.keyword Mitosis
dc.subject.keyword NEDD1
dc.rights.accessRights info:eu-repo/semantics/embargoedAccess
dc.type.version info:eu-repo/semantics/publishedVersion
dc.embargo.liftdate 2020-05-16
dc.date.embargoEnd info:eu-repo/date/embargoEnd/2020-05-16


This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics

Compliant to Partaking