dc.contributor.author |
Alioto, Tyler |
dc.contributor.author |
Cruz, Fernando |
dc.contributor.author |
Gómez Garrido, Jèssica |
dc.contributor.author |
Triyatni, Miriam |
dc.contributor.author |
Gut, Marta |
dc.contributor.author |
Frias, Leonor |
dc.contributor.author |
Esteve-Codina, Anna |
dc.contributor.author |
Menne, Stephan |
dc.contributor.author |
Kiialainen, Anna |
dc.contributor.author |
Kumpesa, Nadine |
dc.contributor.author |
Birzele, Fabian |
dc.contributor.author |
Schmucki, Roland |
dc.contributor.author |
Gut, Ivo Glynne |
dc.contributor.author |
Spleiss, Olivia |
dc.date.accessioned |
2020-03-25T07:35:58Z |
dc.date.available |
2020-03-25T07:35:58Z |
dc.date.issued |
2019 |
dc.identifier.citation |
Alioto TS, Cruz F, Gómez-Garrido J, Triyatni M, Gut M, Frias L, Esteve-Codina A, Menne S, Kiialainen A, Kumpesa N, Birzele F, Schmucki R, Gut IG, Spleiss O. The genome sequence of the eastern woodchuck (Marmota monax) - A preclinical animal model for chronic hepatitis B. G3 (Bethesda). 2019; 9(12):3943-52. DOI: 10.1534/g3.119.400413 |
dc.identifier.issn |
2160-1836 |
dc.identifier.uri |
http://hdl.handle.net/10230/44016 |
dc.description.abstract |
The Eastern woodchuck (Marmota monax) has been extensively used in research of chronic hepatitis B and liver cancer because its infection with the woodchuck hepatitis virus closely resembles a human hepatitis B virus infection. Development of novel immunotherapeutic approaches requires genetic information on immune pathway genes in this animal model. The woodchuck genome was assembled with a combination of high-coverage whole-genome shotgun sequencing of Illumina paired-end, mate-pair libraries and fosmid pool sequencing. The result is a 2.63 Gigabase (Gb) assembly with a contig N50 of 74.5 kilobases (kb), scaffold N50 of 892 kb, and genome completeness of 99.2%. RNA sequencing (RNA-seq) from seven different tissues aided in the annotation of 30,873 protein-coding genes, which in turn encode 41,826 unique protein products. More than 90% of the genes have been functionally annotated, with 82% of them containing open reading frames. This genome sequence and its annotation will enable further research in chronic hepatitis B and hepatocellular carcinoma and contribute to the understanding of immunological responses in the woodchuck. |
dc.format.mimetype |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Genetics Society of America |
dc.relation.ispartof |
G3 (Bethesda). 2019; 9(12):3943-52 |
dc.rights |
© 2019 Alioto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
dc.rights.uri |
http://creativecommons.org/licenses/by/4.0/ |
dc.title |
The genome sequence of the eastern woodchuck (Marmota monax) - A preclinical animal model for chronic hepatitis B |
dc.type |
info:eu-repo/semantics/article |
dc.identifier.doi |
http://dx.doi.org/10.1534/g3.119.400413 |
dc.subject.keyword |
Chronic hepatitis B |
dc.subject.keyword |
Eastern woodchuck |
dc.subject.keyword |
Genome assembly |
dc.subject.keyword |
Hepatocellular carcinoma |
dc.subject.keyword |
Immune response |
dc.subject.keyword |
Marmota monax |
dc.subject.keyword |
Whole genome sequencing |
dc.rights.accessRights |
info:eu-repo/semantics/openAccess |
dc.type.version |
info:eu-repo/semantics/publishedVersion |