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Electrical polarization enables integrative quality control during bacterial differentiation into spores

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dc.contributor.author Sirec, Teja
dc.contributor.author Benarroch, Jonatan M.
dc.contributor.author Buffard, Pauline
dc.contributor.author García Ojalvo, Jordi
dc.contributor.author Asally, Munehiro
dc.date.accessioned 2019-09-05T15:58:09Z
dc.date.available 2019-09-05T15:58:09Z
dc.date.issued 2019
dc.identifier.citation Sirec T, Benarroch JM, Buffard P, Garcia-Ojalvo J, Asally M. Electrical polarization enables integrative quality control during bacterial differentiation into spores. iScience. 2019; 16:378-389. DOI 10.1016/j.isci.2019.05.044
dc.identifier.issn 2589-0042
dc.identifier.uri http://hdl.handle.net/10230/42247
dc.description.abstract Quality control of offspring is important for the survival of cells. However, the mechanisms by which quality of offspring cells may be checked while running genetic programs of cellular differentiation remain unclear. Here we investigated quality control during sporulating in Bacillus subtilis by combining single-cell time-lapse microscopy, molecular biology, and mathematical modeling. Our results revealed that the quality control via premature germination is coupled with the electrical polarization of outer membranes of developing forespores. The forespores that accumulate fewer cations on their surface are more likely to be aborted. This charge accumulation enables the projection of multi-dimensional information about the external environment and morphological development of the forespore into one-dimensional information of cation accumulation. We thus present a paradigm of cellular regulation by bacterial electrical signaling. Moreover, based on the insight we gain, we propose an electrophysiology-based approach of reducing the yield and quality of Bacillus endospores.
dc.description.sponsorship This study was supported by the start-up fund from University of Warwick, SLS Pump priming fund, and the Royal Society Research Grant to M.A., BBSRC/EPSRC grant to WISB (BB/M017982/1), and the Spanish Ministry of Economy and Competitiveness and FEDER (FIS2015-66503-C3-1-P) and the Maria de Maeztu Program for Units of Excellence (project MDM-2014-0370) to J.G.-O. J.M.B. acknowledges the funding by the MRC Doctoral Training Partnership (MR/N014294/1). P.B. was supported by a scholarship from the Region Auvergne-Rhone-Alpes and the Université Grenoble Alpes.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof iScience. 2019; 16:378-389
dc.rights © 2019 The Author(s).This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.title Electrical polarization enables integrative quality control during bacterial differentiation into spores
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.isci.2019.05.044
dc.subject.keyword Microbiology
dc.subject.keyword Microbial Physiology
dc.subject.keyword Bioinformatics
dc.subject.keyword Mathematical Biosciences
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/FIS2015-66503-C3-1-P
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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