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Estimation of cell proliferation dynamics using CFSE data

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dc.contributor.author Banks, H. Thomas
dc.contributor.author Sutton, Karyn L.
dc.contributor.author Thompson, W. Clayton
dc.contributor.author Bocharov, Gennady A.
dc.contributor.author Roose, Dirk
dc.contributor.author Schenkel, Tim
dc.contributor.author Meyerhans, Andreas
dc.date.accessioned 2019-02-21T08:35:19Z
dc.date.available 2019-02-21T08:35:19Z
dc.date.issued 2011
dc.identifier.citation Banks HT, Sutton KL, Thompson WC, Bocharov G, Roose D, Schenkel T et al. Estimation of cell proliferation dynamics using CFSE data. Bull Math Biol. 2011;73(1):116-50. DOI: 10.1007/s11538-010-9524-5
dc.identifier.issn 0092-8240
dc.identifier.uri http://hdl.handle.net/10230/36642
dc.description.abstract Advances in fluorescent labeling of cells as measured by flow cytometry have allowed for quantitative studies of proliferating populations of cells. The investigations (Luzyanina et al. in J. Math. Biol. 54:57-89, 2007; J. Math. Biol., 2009; Theor. Biol. Med. Model. 4:1-26, 2007) contain a mathematical model with fluorescence intensity as a structure variable to describe the evolution in time of proliferating cells labeled by carboxyfluorescein succinimidyl ester (CFSE). Here, this model and several extensions/modifications are discussed. Suggestions for improvements are presented and analyzed with respect to statistical significance for better agreement between model solutions and experimental data. These investigations suggest that the new decay/label loss and time dependent effective proliferation and death rates do indeed provide improved fits of the model to data. Statistical models for the observed variability/noise in the data are discussed with implications for uncertainty quantification. The resulting new cell dynamics model should prove useful in proliferation assay tracking and modeling, with numerous applications in the biomedical sciences.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof Bulletin of Mathematical Biology. 2011;73(1):116-50
dc.rights © Springer The final publication is available at Springer via http://dx.doi.org/10.1007/s11538-010-9524-5.
dc.title Estimation of cell proliferation dynamics using CFSE data
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1007/s11538-010-9524-5
dc.subject.keyword Cell proliferation
dc.subject.keyword CFSE
dc.subject.keyword Label structured population dynamics
dc.subject.keyword Partial differential equations
dc.subject.keyword Inverse problems
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/acceptedVersion


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