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Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9

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dc.contributor.author Niu, Dong
dc.contributor.author Wei, Hong-Jiang
dc.contributor.author Lin, Lin
dc.contributor.author George, Haydy
dc.contributor.author Wang, Tao
dc.contributor.author Lee, I-Hsiu
dc.contributor.author Zhao, Hong-Ye
dc.contributor.author Wang, Yong
dc.contributor.author Kan, Yinan
dc.contributor.author Shrock, Ellen
dc.contributor.author Lesha, Emal
dc.contributor.author Wang, Gang
dc.contributor.author Luo, Yonglun
dc.contributor.author Qing, Yubo
dc.contributor.author Jiao, Deling
dc.contributor.author Zhao, Heng
dc.contributor.author Zhou, Xiaoyang
dc.contributor.author Wang, Shouqi
dc.contributor.author Wei, Hong-Jiang
dc.contributor.author Güell Cargol, Marc
dc.contributor.author Church, George M.
dc.contributor.author Yang, Luhan
dc.date.accessioned 2018-11-14T14:34:30Z
dc.date.available 2018-11-14T14:34:30Z
dc.date.issued 2017
dc.identifier.citation Niu D, Wei HJ, Lin L, George H, Wang T, Lee IH et al. Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9. Science. 2017 Sep 22;357(6357):1303-7. DOI: 10.1126/science.aan4187
dc.identifier.issn 0036-8075
dc.identifier.uri http://hdl.handle.net/10230/35752
dc.description.abstract Xenotransplantation is a promising strategy to alleviate the shortage of organs for human transplantation. In addition to the concerns about pig-to-human immunological compatibility, the risk of cross-species transmission of porcine endogenous retroviruses (PERVs) has impeded the clinical application of this approach. We previously demonstrated the feasibility of inactivating PERV activity in an immortalized pig cell line. We now confirm that PERVs infect human cells, and we observe the horizontal transfer of PERVs among human cells. Using CRISPR-Cas9, we inactivated all of the PERVs in a porcine primary cell line and generated PERV-inactivated pigs via somatic cell nuclear transfer. Our study highlights the value of PERV inactivation to prevent cross-species viral transmission and demonstrates the successful production of PERV-inactivated animals to address the safety concern in clinical xenotransplantation.
dc.description.sponsorship This study is mainly funded by eGenesis Inc. and was funded by NIH grant P50 HG005550. Y.L. was funded by Danish Research Council for Independent Research (DFF-1337-00128) and Sapere Aude Young Research Talent Prize (DFF-1335-00763). M.G. was funded by a Human Frontiers Science Program Long Term fellowship. Some of the pig production was funded by Major Program on Basic Research Projects of Yunnan Province, China (Grant No. 2014FC006). PERV elements genotyping illumina miseq data have been uploaded to the European Nucleotide Archive (ENA) hosted by the European Bioinformatics Institute (EBI) with the submission reference PRJEB11222
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher American Association for the Advancement of Science (AAAS)
dc.relation.ispartof Science. 2017 Sep 22;357(6357):1303-7
dc.rights This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science on Vol. 357 Issue 6357, 22 Sep 2017. DOI: 10.1126/science.aan4187
dc.subject.other Xenotrasplantament
dc.subject.other Malalties transmissibles
dc.subject.other Silenciament gènic
dc.title Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1126/science.aan4187
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/acceptedVersion


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