Welcome to the UPF Digital Repository

A Clb/Cdk1-mediated regulation of Fkh2 synchronizes CLB expression in the budding yeast cell cycle

Show simple item record

dc.contributor.author Linke, Christian
dc.contributor.author Chasapi, Anastasia
dc.contributor.author González Novo, Alberto
dc.contributor.author Al-Sawad, Istabrak
dc.contributor.author Tognetti, Silvia
dc.contributor.author Klipp, Edda
dc.contributor.author Loog, Mart
dc.contributor.author Krobitsch, Sylvia
dc.contributor.author Posas Garriga, Francesc
dc.contributor.author Xenarios, Ioannis
dc.contributor.author Barberis, Matteo
dc.date.accessioned 2018-11-12T11:40:09Z
dc.date.available 2018-11-12T11:40:09Z
dc.date.issued 2017
dc.identifier.citation Linke C, Chasapi A, González-Novo A, Al Sawad I, Tognetti S, Klipp E et al. A Clb/Cdk1-mediated regulation of Fkh2 synchronizes CLB expression in the budding yeast cell cycle. NPJ Syst Biol Appl. 2017 Mar 6; 3: 7. DOI: 10.1038/s41540-017-0008-1
dc.identifier.issn 2056-7189
dc.identifier.uri http://hdl.handle.net/10230/35731
dc.description.abstract Precise timing of cell division is achieved by coupling waves of cyclin-dependent kinase (Cdk) activity with a transcriptional oscillator throughout cell cycle progression. Although details of transcription of cyclin genes are known, it is unclear which is the transcriptional cascade that modulates their expression in a timely fashion. Here, we demonstrate that a Clb/Cdk1-mediated regulation of the Fkh2 transcription factor synchronizes the temporal mitotic CLB expression in budding yeast. A simplified kinetic model of the cyclin/Cdk network predicts a linear cascade where a Clb/Cdk1-mediated regulation of an activator molecule drives CLB3 and CLB2 expression. Experimental validation highlights Fkh2 as modulator of CLB3 transcript levels, besides its role in regulating CLB2 expression. A Boolean model based on the minimal number of interactions needed to capture the information flow of the Clb/Cdk1 network supports the role of an activator molecule in the sequential activation, and oscillatory behavior, of mitotic Clb cyclins. This work illustrates how transcription and phosphorylation networks can be coupled by a Clb/Cdk1-mediated regulation that synchronizes them.
dc.description.sponsorship This work was supported by the SILS Starting Grant of the University of Amsterdam, UvA to M.B., by the UvA-Systems Biology Research Priority Area grant to M.B., and by the ENFIN Network of Excellence grant funded by the European Commission to M.B. (within contract number LSHG-CT-2005-518254 to E.K.). We also acknowledge the grant from the European Commission UNICELLSYS, contract number HEALTH-2007-201142 to E.K., S.K., and F.P.); the Spanish Ministry of Economy and Competitiveness (BFU2012-33503 and FEDER) and by Fundación Botín, by Banco Santander through its Santander Universities Global Division to F.P.; the SNSF Swiss National Science Foundation (contract number 132392, SYNERGIA) and State Secretariat for Science Research and Innovation (SEFRI) to I.X.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Nature Publishing Group
dc.rights © Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.other Cicle cel·lular
dc.title A Clb/Cdk1-mediated regulation of Fkh2 synchronizes CLB expression in the budding yeast cell cycle
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1038/s41540-017-0008-1
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP6/518254
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/201142
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-33503
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search


My Account


Compliant to Partaking