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Quantitative measurements in 3-dimensional datasets of mouse lymph nodes resolve organ-wide functional dependencies

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dc.contributor.author Mayer, Jürgen, 1977-
dc.contributor.author Swoger, Jim
dc.contributor.author Ozga, Aleksandra J.
dc.contributor.author Stein, Jens V.
dc.contributor.author Sharpe, James
dc.date.accessioned 2018-07-19T07:33:20Z
dc.date.available 2018-07-19T07:33:20Z
dc.date.issued 2012
dc.identifier.citation Mayer J, Swoger J, Ozga AJ, Stein JV, Sharpe J. Quantitative measurements in 3-dimensional datasets of mouse lymph nodes resolve organ-wide functional dependencies. Comput Math Methods Med. 2012; 2012: 128431. DOI: 10.1155/2012/128431
dc.identifier.issn 1748-670X
dc.identifier.uri http://hdl.handle.net/10230/35195
dc.description.abstract Deep tissue imaging has become state of the art in biology, but now the problem is to quantify spatial information in a global, organ-wide context. Although access to the raw data is no longer a limitation, the computational tools to extract biologically useful information out of these large data sets is still catching up. In many cases, to understand the mechanism behind a biological process, where molecules or cells interact with each other, it is mandatory to know their mutual positions. We illustrate this principle here with the immune system. Although the general functions of lymph nodes as immune sentinels are well described, many cellular and molecular details governing the interactions of lymphocytes and dendritic cells remain unclear to date and prevent an in-depth mechanistic understanding of the immune system. We imaged ex vivo lymph nodes isolated from both wild-type and transgenic mice lacking key factors for dendritic cell positioning and used software written in MATLAB to determine the spatial distances between the dendritic cells and the internal high endothelial vascular network. This allowed us to quantify the spatial localization of the dendritic cells in the lymph node, which is a critical parameter determining the effectiveness of an adaptive immune response.
dc.description.sponsorship The authors are grateful for funding from VIBRANT Grant CP-IP 228933-2 from the FP7-NMP and Sinergia Grant CRSII3 125447 from the Swiss National Science Foundation (SNSF)
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Hindawi
dc.rights Copyright © 2012 Jürgen Mayer et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject.other Nodes limfàtics
dc.title Quantitative measurements in 3-dimensional datasets of mouse lymph nodes resolve organ-wide functional dependencies
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1155/2012/128431
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/228933
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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