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Automatic analysis and 3D-modelling of Hi-C data using TADbit reveals structural features of the fly chromatin colors

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dc.contributor.author Serra, François
dc.contributor.author Baù, Davide
dc.contributor.author Goodstadt, Michael
dc.contributor.author Castillo Andreo, David
dc.contributor.author Filion, Guillaume
dc.contributor.author Martí Renom, Marc A.
dc.date.accessioned 2018-07-04T07:56:30Z
dc.date.available 2018-07-04T07:56:30Z
dc.date.issued 2017
dc.identifier.citation Serra F, Baù D, Goodstadt M, Castillo D, Filion GJ, Marti-Renom MA. Automatic analysis and 3D-modelling of Hi-C data using TADbit reveals structural features of the fly chromatin colors. PLoS Comput Biol. 2017 Jul 19;13(7):e1005665. DOI: 10.1371/journal.pcbi.1005665
dc.identifier.issn 1553-734X
dc.identifier.uri http://hdl.handle.net/10230/35026
dc.description.abstract The sequence of a genome is insufficient to understand all genomic processes carried out in the cell nucleus. To achieve this, the knowledge of its three-dimensional architecture is necessary. Advances in genomic technologies and the development of new analytical methods, such as Chromosome Conformation Capture (3C) and its derivatives, provide unprecedented insights in the spatial organization of genomes. Here we present TADbit, a computational framework to analyze and model the chromatin fiber in three dimensions. Our package takes as input the sequencing reads of 3C-based experiments and performs the following main tasks: (i) pre-process the reads, (ii) map the reads to a reference genome, (iii) filter and normalize the interaction data, (iv) analyze the resulting interaction matrices, (v) build 3D models of selected genomic domains, and (vi) analyze the resulting models to characterize their structural properties. To illustrate the use of TADbit, we automatically modeled 50 genomic domains from the fly genome revealing differential structural features of the previously defined chromatin colors, establishing a link between the conformation of the genome and the local chromatin composition. TADbit provides three-dimensional models built from 3C-based experiments, which are ready for visualization and for characterizing their relation to gene expression and epigenetic states. TADbit is an open-source Python library available for download from https://github.com/3DGenomes/tadbit.
dc.description.sponsorship The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013) / ERC grant agreement 609989, the Spanish Ministry of Economy and Competitiveness (BFU2013-47736-P) and the Human Frontiers Science Program (RGP0044). We acknowledge support of the CERCA Programme / Generalitat de Catalunya and the Spanish Ministry of Economy and Competitiveness, 'Centro de Excelencia Severo Ochoa 2013-2017', SEV-2012-0208 to the CRG.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PLOS Computational Biology. 2017 Jul 19;13(7):e1005665
dc.rights © 2017 Serra et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Automatic analysis and 3D-modelling of Hi-C data using TADbit reveals structural features of the fly chromatin colors
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1371/journal.pcbi.1005665
dc.subject.keyword Chromatin
dc.subject.keyword Drosophila melanogaster
dc.subject.keyword Chromosome mapping
dc.subject.keyword Structural genomics
dc.subject.keyword Invertebrate genomics
dc.subject.keyword Mammalian genomics
dc.subject.keyword Genome analysis
dc.subject.keyword Genomic libraries
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/609989
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2013-47736-P
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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