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dc.contributor.author | Stadhouders, Ralph |
dc.contributor.author | Vidal Ocabo, Enrique |
dc.contributor.author | Serra, François |
dc.contributor.author | Di Stefano, Bruno, 1984- |
dc.contributor.author | Le Dily, François |
dc.contributor.author | Quilez Oliete, Javier |
dc.contributor.author | Gómez, Antonio |
dc.contributor.author | Collombet, Samuel |
dc.contributor.author | Berenguer, Clara |
dc.contributor.author | Cuartero, Yasmina |
dc.contributor.author | Hecht, Jochen |
dc.contributor.author | Filion, Guillaume |
dc.contributor.author | Beato, Miguel |
dc.contributor.author | Martí Renom, Marc A. |
dc.contributor.author | Graf, T. (Thomas) |
dc.date.accessioned | 2018-06-14T07:43:14Z |
dc.date.issued | 2018 |
dc.identifier.citation | Stadhouders R, Vidal E, Serra F, Di Stefano B, Le Dily F, Quilez J et al. Transcription factors orchestrate dynamic interplay between genome topology and gene regulation during cell reprogramming. Nat Genet. 2018 Feb;50(2):238-49. DOI: 10.1038/s41588-017-0030-7. |
dc.identifier.issn | 1061-4036 |
dc.identifier.uri | http://hdl.handle.net/10230/34895 |
dc.description.abstract | Chromosomal architecture is known to influence gene expression, yet its role in controlling cell fate remains poorly understood. Reprogramming of somatic cells into pluripotent stem cells (PSCs) by the transcription factors (TFs) OCT4, SOX2, KLF4 and MYC offers an opportunity to address this question but is severely limited by the low proportion of responding cells. We have recently developed a highly efficient reprogramming protocol that synchronously converts somatic into pluripotent stem cells. Here, we used this system to integrate time-resolved changes in genome topology with gene expression, TF binding and chromatin-state dynamics. The results showed that TFs drive topological genome reorganization at multiple architectural levels, often before changes in gene expression. Removal of locus-specific topological barriers can explain why pluripotency genes are activated sequentially, instead of simultaneously, during reprogramming. Together, our results implicate genome topology as an instructive force for implementing transcriptional programs and cell fate in mammals. |
dc.description.sponsorship | This work was supported by the European Research Council under the 7th Framework Programme FP7/2007-2013 (ERC Synergy Grant 4D-Genome, grant agreement 609989 to T.G., G.J.F., M.A.M.-R. and M.B.) and the Ministerio de Educacion y Ciencia, SAF.2012-37167. R.S. was supported by an EMBO Long-term Fellowship (ALTF 1201-2014) and a Marie Curie Individual Fellowship (H2020-MSCA-IF-2014). We also acknowledge support from 'Centro de Excelencia Severo Ochoa 2013-2017' (SEV-2012-0208) and AGAUR to the CRG. |
dc.format.mimetype | application/pdf |
dc.language.iso | eng |
dc.publisher | Nature Publishing Group |
dc.relation.ispartof | Nature Genetics. 2018 Feb;50(2):238-49 |
dc.rights | © Nature Publishing Group. http://dx.doi.org/10.1038/s41588-017-0030-7 |
dc.title | Transcription factors orchestrate dynamic interplay between genome topology and gene regulation during cell reprogramming |
dc.type | info:eu-repo/semantics/article |
dc.identifier.doi | http://dx.doi.org/10.1038/s41588-017-0030-7 |
dc.subject.keyword | Gene expression regulation |
dc.subject.keyword | Cellular reprogramming |
dc.subject.keyword | Induced pluripotent stem cells |
dc.subject.keyword | Diploid cell |
dc.subject.keyword | Klf4 gene |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/609989 |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/3PN/SAF2012-37167 |
dc.rights.accessRights | info:eu-repo/semantics/openAccess |
dc.type.version | info:eu-repo/semantics/acceptedVersion |