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Evaluation of different peptide fragmentation types and mass analyzers in data-dependent methods using an Orbitrap Fusion Lumos Tribrid mass spectrometer

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dc.contributor.author Espadas, Guadalupe
dc.contributor.author Borràs, Eva
dc.contributor.author Chiva, Cristina
dc.contributor.author Sabidó Aguadé, Eduard, 1981-
dc.date.accessioned 2018-06-07T09:47:32Z
dc.date.available 2018-06-07T09:47:32Z
dc.date.issued 2017
dc.identifier.citation Espadas G, Borràs E, Chiva C, Sabidó E. Evaluation of different peptide fragmentation types and mass analyzers in data-dependent methods using an Orbitrap Fusion Lumos Tribrid mass spectrometer. Proteomics. 2017 May;17(9):1600416. DOI: 10.1002/pmic.201600416
dc.identifier.issn 1615-9853
dc.identifier.uri http://hdl.handle.net/10230/34853
dc.description.abstract One of the major additions in MS technology has been the irruption of the Orbitrap mass analyzer, which has boosted the proteomics analyses of biological complex samples since its introduction. Here, we took advantage of the capabilities of the new Orbitrap Fusion Lumos Tribrid mass spectrometer to assess the performance of different data-dependent acquisition methods for the identification and quantitation of peptides and phosphopeptides in single-shot analysis of human whole cell lysates. Our study explored the capabilities of tri-hibrid mass spectrometers for (phospho-) peptide identification and quantitation using different gradient lengths, sample amounts, and combinations of different peptide fragmentation types and mass analyzers. Moreover, the acquisition of the same complex sample with different acquisition methods resulted in the generation of a dataset to be used as a reference for further analyses, and a starting point for future optimizations in particular applications.
dc.description.sponsorship The CRG/UPF Proteomics Unit is part of the “Plataforma de Recursos Biomoleculares y Bioinformáticos (ProteoRed)” supported by grant PT13/0001 of ISCIII and Spanish Ministry of Economy and Competitiveness. We acknowledge support of the Spanish Ministry of Economy and Competitiveness, “Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016” (CTQ2016‐80364‐P) “Centro de Excelencia Severo Ochoa 2013-2017” (SEV‐2012‐0208), and from “Secretaria d'Universitats i Recerca del Departament d'Economia i Coneixement de la Generalitat de Catalunya” (2014SGR678).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartof Proteomics. 2017 May;17(9):1600416
dc.rights This is the peer reviewed version of the following article: Espadas G, Borràs E, Chiva C, Sabidó E. Evaluation of different peptide fragmentation types and mass analyzers in data-dependent methods using an Orbitrap Fusion Lumos Tribrid mass spectrometer. Proteomics. 2017 May;17(9):1600416, which has been published in final form at http://dx.doi.org/10.1002/pmic.201600416. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
dc.title Evaluation of different peptide fragmentation types and mass analyzers in data-dependent methods using an Orbitrap Fusion Lumos Tribrid mass spectrometer
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1002/pmic.201600416
dc.subject.keyword Mass spectrometry
dc.subject.keyword Phosphorylation
dc.subject.keyword Protein identification
dc.subject.keyword Protein quantitation
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/CTQ2016-80364-P
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/acceptedVersion


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