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3D modeling of chromatin structure: is there a way to integrate and reconcile single cell and population experimental data?

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dc.contributor.author Le Dily, François
dc.contributor.author Serra, François
dc.contributor.author Marti-Renom, Marc A.
dc.date.accessioned 2017-05-17T08:40:27Z
dc.date.issued 2017
dc.identifier.citation Le Dily F, Serra F, Martí Renom MA. 3D modeling of chromatin structure: is there a way to integrate and reconcile single cell and population experimental data? Wiley Interdiscip Rev Comput Mol Sci. 2017 April 10. [Epub ahead of print]. DOI: 10.1002/wcms.1308
dc.identifier.issn 1759-0876
dc.identifier.uri http://hdl.handle.net/10230/32133
dc.description.abstract The genome is organized in a hierarchical fashion within the nucleus in interphase. This nonrandom folding of the chromatin fiber is thought to play important roles in the processing of the genetic information. Therefore, a better knowledge of the mechanisms underlying the three-dimensional structure of the genome appears essential to fully understand the nuclear processes including transcription and replication. Fluorescent in situ hybridization (FISH) and molecular biology methods deriving from the Chromosome Conformation Capture technique are the methods of choice to study genome 3D organization at different levels. Although these single cell and population methods allowed to highlight similar chromatin structures, they also show frequent discrepancies which might be better understood by improving the capacity to generate actual 3D models of organization based on the different types of data available. This review aims at giving an overview of the principles, advantages, and limits of microscopy and molecular biology methods of analysis of genome structure and at discussing the different approaches of modeling of chromatin classically used and the improvements that are necessary to reach a better understanding on the links between chromatin structure and its spatial organization.
dc.description.sponsorship This review has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement 609989. We acknowledge funding from the Spanish Ministry of Economy and Competitiveness (BFU2013-47736-P) and the European Union's Horizon 2020 research and innovation programme under grant agreement No 676556 and support of the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Severo Ochoa 2013-2017’, SEV-2012-0208 and of the CERCA Programme/Generalitat de Catalunya to the CRG
dc.language.iso eng
dc.publisher Wiley
dc.rights This is the peer reviewed version of the following article: Le Dily F, Serra F, Martí Renom MA. 3D modeling of chromatin structure: is there a way to integrate and reconcile single cell and population experimental data? Wiley Interdiscip Rev Comput Mol Sci. 2017 April 10. [Epub ahead of print], which has been published in final form at http://dx.doi.org/10.1002/wcms.1308. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
dc.subject.other Cromatina -- Estructura
dc.subject.other Genètica
dc.subject.other Genomes
dc.title 3D modeling of chromatin structure: is there a way to integrate and reconcile single cell and population experimental data?
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1002/wcms.1308
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/609989
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/676556
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2013-47736-P
dc.rights.accessRights info:eu-repo/semantics/embargoedAccess
dc.type.version info:eu-repo/semantics/acceptedVersion
dc.embargo.liftdate 2018-04-10
dc.date.embargoEnd info:eu-repo/date/embargoEnd/2018-04-10

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