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Insights into the Mechanisms of Basal Coordination of Transcription Using a Genome-Reduced Bacterium

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dc.contributor.author Junier, Ivan
dc.contributor.author Unal, E. Besray
dc.contributor.author Yus, Eva
dc.contributor.author Lloréns Rico, Verónica, 1989-
dc.contributor.author Serrano Pubull, Luis, 1982-
dc.date.accessioned 2017-04-19T07:21:16Z
dc.date.available 2017-04-19T07:21:16Z
dc.date.issued 2016
dc.identifier.citation Junier I, Unal EB, Yus E, Lloréns Rico V, Serrano Pubull L. Insights into the Mechanisms of Basal Coordination of Transcription Using a Genome-Reduced Bacterium. Cell Systems. 2016; 2(6): 391-401. DOI: 10.1016/j.cels.2016.04.015
dc.identifier.issn 2405-4720
dc.identifier.uri http://hdl.handle.net/10230/30836
dc.description.abstract Coordination of transcription in bacteria occurs at supra-operonic scales, but the extent, specificity, and mechanisms of such regulation are poorly understood. Here, we tackle this problem by profiling the transcriptome of the model organism Mycoplasma pneumoniae across 115 growth conditions. We identify three qualitatively different levels of co-expression corresponding to distinct relative orientations and intergenic properties of adjacent genes. We reveal that the degree of co-expression between co-directional adjacent operons, and more generally between genes, is tightly related to their capacity to be transcribed en bloc into the same mRNA. We further show that this genome-wide pervasive transcription of adjacent genes and operons is specifically repressed by DNA regions preferentially bound by RNA polymerases, by intrinsic terminators, and by large intergenic distances. Taken together, our findings suggest that the basal coordination of transcription is mediated by the physical entities and mechanical properties of the transcription process itself, and that operon-like behaviors may strongly vary from condition to condition.
dc.description.sponsorship This work was supported by Fundación Marcelino Botin and the Spanish Ministerio de Economía y Competitividad (BIO2007-61762). This project was financed by Instituto de Salud Carlos III and co-financed by Federación Española de Enfermedades Raras under grant agreement PI10/01702 and the European Research Council and European Union’s Horizon 2020 research and innovation program under grant agreements 634942 (MycoSynVac) and 670216 (MYCOCHASSIS). The Centre for Genomic Regulation acknowledges the support of the Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa 2013-2017,” SEV-2012-0208.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof Cell Systems. 2016; 2(6): 391-401
dc.rights Cell Systems 2, 391–401, June 22, 2016. © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.title Insights into the Mechanisms of Basal Coordination of Transcription Using a Genome-Reduced Bacterium
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.cels.2016.04.015
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/634942
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/670216
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SEV2012-0208
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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