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Q-nexus: a comprehensive and efficient analysis pipeline designed for ChIP-nexus

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dc.contributor.author Hansen, Peter
dc.contributor.author Hecht, Jochen
dc.contributor.author Ibn-Salem, Jonas
dc.contributor.author Menkuec, Benjamin S.
dc.contributor.author Roskosch, Sebastian
dc.contributor.author Truss, Matthias
dc.contributor.author Robinson, Peter N.
dc.date.accessioned 2017-01-12T11:18:49Z
dc.date.available 2017-01-12T11:18:49Z
dc.date.issued 2016
dc.identifier.citation Hansen P, Hecht J, Ibn-Salem J, Menkuec BS, Roskosch S, Truss M, Robinson PN. Q-nexus: a comprehensive and efficient analysis pipeline designed for ChIP-nexus. BMC Genomics. 2016;17:873. DOI: 10.1186/s12864-016-3164-6
dc.identifier.issn 1471-2164
dc.identifier.uri http://hdl.handle.net/10230/27873
dc.description.abstract Background: ChIP-nexus, an extension of the ChIP-exo protocol, can be used to map the borders of protein-bound DNA sequences at nucleotide resolution, requires less input DNA and enables selective PCR duplicate removal using random barcodes. However, the use of random barcodes requires additional preprocessing of the mapping data, which complicates the computational analysis. To date, only a very limited number of software packages are available for the analysis of ChIP-exo data, which have not yet been systematically tested and compared on ChIP-nexus data. Results: Here, we present a comprehensive software package for ChIP-nexus data that exploits the random barcodes for selective removal of PCR duplicates and for quality control. Furthermore, we developed bespoke methods to estimate the width of the protected region resulting from protein-DNA binding and to infer binding positions from ChIP-nexus data. Finally, we applied our peak calling method as well as the two other methods MACE and MACS2 to the available ChIP-nexus data. Conclusions: The Q-nexus software is efficient and easy to use. Novel statistics about duplication rates in consideration of random barcodes are calculated. Our method for the estimation of the width of the protected region yields unbiased signatures that are highly reproducible for biological replicates and at the same time very specific for the respective factors analyzed. As judged by the irreproducible discovery rate (IDR), our peak calling algorithm shows a substantially better reproducibility. An implementation of Q-nexus is available at http://charite.github.io/Q/.
dc.description.sponsorship This project was supported by the Bundesministerium für Bildung und Forschung (BMBF; project no. 0313911 and 13GW0099) and the European Community’s Seventh Framework Programme (grant agreement no. 602300; SYBIL). Furthermore, we acknowledge support of the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Severo Ochoa 2013-2017’.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.ispartof BMC Genomics. 2016;17:873
dc.rights © The Author(s). 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to theCreative Commons license, and indicate if changes were made.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Q-nexus: a comprehensive and efficient analysis pipeline designed for ChIP-nexus
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1186/s12864-016-3164-6
dc.subject.keyword Chromatin immunoprecipitation
dc.subject.keyword ChIP-nexus
dc.subject.keyword ChIP-exo
dc.subject.keyword Duplication rates
dc.subject.keyword Library complexity
dc.subject.keyword Algorithm
dc.subject.keyword Bioinformatics
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/602300
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SEV2012-0208
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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