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Time-course of muscle mass loss, Damage, and proteolysis in gastrocnemius following unloading and reloading: implications in chronic diseases.

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dc.contributor.author Chacón-Cabrera, Alba
dc.contributor.author Lund-Palau, Helena
dc.contributor.author Gea Guiral, Joaquim
dc.contributor.author Barreiro Portela, Esther
dc.date.accessioned 2016-11-22T11:10:40Z
dc.date.available 2016-11-22T11:10:40Z
dc.date.issued 2016
dc.identifier.citation Chacon-Cabrera A, Lund-Palau H, Gea J, Barreiro E. Time-Course of Muscle Mass Loss, Damage, and Proteolysis in Gastrocnemius following Unloading and Reloading: Implications in Chronic Diseases. PLoS One. 2016 Oct 28;11(10):e0164951. DOI: 10.1371/journal.pone.0164951
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10230/27561
dc.description.abstract BACKGROUND: Disuse muscle atrophy is a major comorbidity in patients with chronic diseases including cancer. We sought to explore the kinetics of molecular mechanisms shown to be involved in muscle mass loss throughout time in a mouse model of disuse muscle atrophy and recovery following immobilization. METHODS: Body and muscle weights, grip strength, muscle phenotype (fiber type composition and morphometry and muscle structural alterations), proteolysis, contractile proteins, systemic troponin I, and mitochondrial content were assessed in gastrocnemius of mice exposed to periods (1, 2, 3, 7, 15 and 30 days) of non-invasive hindlimb immobilization (plastic splint, I cohorts) and in those exposed to reloading for different time-points (1, 3, 7, 15, and 30 days, R cohorts) following a seven-day period of immobilization. Groups of control animals were also used. RESULTS: Compared to non-exposed controls, muscle weight, limb strength, slow- and fast-twitch cross-sectional areas, mtDNA/nDNA, and myosin content were decreased in mice of I cohorts, whereas tyrosine release, ubiquitin-proteasome activity, muscle injury and systemic troponin I levels were increased. Gastrocnemius reloading following splint removal improved muscle mass loss, strength, fiber atrophy, injury, myosin content, and mtDNA/nDNA, while reducing ubiquitin-proteasome activity and proteolysis. CONCLUSIONS: A consistent program of molecular and cellular events leading to reduced gastrocnemius muscle mass and mitochondrial content and reduced strength, enhanced proteolysis, and injury, was seen in this non-invasive mouse model of disuse muscle atrophy. Unloading of the muscle following removal of the splint significantly improved the alterations seen during unloading, characterized by a specific kinetic profile of molecular events involved in muscle regeneration. These findings have implications in patients with chronic diseases including cancer in whom physical activity may be severely compromised.
dc.description.sponsorship This study has been supported by the following Spanish agencies: CIBERES, PI14/00713 (FEDER), and SAF-2014-54371-R (FEDER) from the Instituto de Salud Carlos III (http://www.isciii.es/); SEPAR 2013 and SEPAR 2016 from Spanish Respiratory Society (SEPAR, http://www.separ.es/); and Catalan Foundation of Pneumology (FUCAP, http://www.ccfundacions.cat/fundacions/fundacio-catalana-de-pneumologia-fucap), FUCAP 2016
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartof PLoS One. 2016 Oct 28;11(10):e0164951
dc.rights © 2016 Chacon-Cabrera et al/nThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject.other Músculs
dc.subject.other Malalties cròniques
dc.title Time-course of muscle mass loss, Damage, and proteolysis in gastrocnemius following unloading and reloading: implications in chronic diseases.
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0164951
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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