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Dependence of electroporation detection threshold on cell radius: an explanation to observations non compatible with Schwan’s equation model

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dc.contributor.author Mercadal, Borja
dc.contributor.author Vernier, P. Thomas
dc.contributor.author Ivorra Cano, Antoni, 1974-
dc.date.accessioned 2016-11-16T10:56:52Z
dc.date.available 2016-11-16T10:56:52Z
dc.date.issued 2016
dc.identifier.citation Mercadal B, Vernier PT, Ivorra A. Dependence of electroporation detection threshold on cell radius: an explanation to observations non compatible with Schwan’s equation model. J Membr Biol. 2016 Oct;249(5):663-76. DOI: 10.1007/s00232-016-9907-0
dc.identifier.issn 0022-2631
dc.identifier.uri http://hdl.handle.net/10230/27523
dc.description.abstract It is widely accepted that electroporation occurs when the cell transmembrane voltage induced by an external applied electric field reaches a threshold. Under this assumption, in order to trigger electroporation in a spherical cell, Schwan’s equation leads to an inversely proportional relationship between the cell radius and the minimum magnitude of the applied electric field. And, indeed, several publications report experimental evidences of an inverse relationship between the cell size and the field required to achieve electroporation. However, this dependence is not always observed or is not as steep as predicted by Schwan’s equation. The present numerical study attempts to explain these observations that do not fit Schwan’s equation on the basis of the interplay between cell membrane conductivity, permeability, and transmembrane voltage. For that, a single cell in suspension was modeled and the electric field necessary to achieve electroporation with a single pulse was determined according to two effectiveness criteria: a specific permeabilization level, understood as the relative area occupied by the pores during the pulse, and a final intracellular concentration of a molecule due to uptake by diffusion after the pulse, during membrane resealing. The results indicate that plausible model parameters can lead to divergent dependencies of the electric field threshold on the cell radius. These divergent dependencies were obtained through both criteria and using two different permeabilization models. This suggests that the interplay between cell membrane conductivity, permeability, and transmembrane voltage might be the cause of results which are noncompatible with the Schwan’s equation model.
dc.description.sponsorship This work was supported by the Ministry of Economy and Competitiveness of Spain through Grant TEC2014-52383-C3-2-R. PTV received support from the Old Dominion University Frank Reidy Research Center for Bioelectrics and the Air Force Office of Scientific Research (FA9550-15-1-0517, FA9550-14-1-0123).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof The Journal of Membrane Biology. 2016;249(5):663-76.
dc.rights © Springer. The final publication is available at Springer via/nhttp://dx.doi.org/10.1007/s00232-016-9907-0
dc.title Dependence of electroporation detection threshold on cell radius: an explanation to observations non compatible with Schwan’s equation model
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1007/s00232-016-9907-0
dc.subject.keyword Electroporation
dc.subject.keyword Cell size
dc.subject.keyword Finite element modeling
dc.subject.keyword Cell membrane
dc.subject.keyword Transmembrane transport
dc.subject.keyword Membrane conductivity
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/TEC2014-52383-C3-2-R
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/acceptedVersion

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