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Detection and characterization of clostebol sulfate metabolites in Caucasian population.

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dc.contributor.author Balcells Aribau, Georgina, 1987-
dc.contributor.author Pozo Mendoza, Óscar J., 1975-
dc.contributor.author Garrostas, Lorena
dc.contributor.author Esquivel López, Argitxu, 1989-
dc.contributor.author Matabosch Geronès, Xavier
dc.contributor.author Kotronoulas, Aristotelis
dc.contributor.author Joglar Tamargo, Jesús
dc.contributor.author Ventura Alemany, Rosa
dc.date.accessioned 2016-07-19T11:10:31Z
dc.date.issued 2016
dc.identifier.citation Balcells G, Pozo OJ, Garrostas L, Esquivel A, Matabosch X, Kotronoulas A. Detection and characterization of clostebol sulfate metabolites in Caucasian population. J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Jun 1;1022:54-63. DOI: 10.1016/j.jchromb.2016.03.028
dc.identifier.issn 1570-0232
dc.identifier.uri http://hdl.handle.net/10230/27090
dc.description.abstract Anabolic androgenic steroids (AAS) are synthetic testosterone derivatives which undergo extensive metabolism in man. Differences in the excretion of phase II metabolites are strongly associated with inter-individual and inter-ethnic variations. Sulfate metabolites have been described as long-term metabolites for some AAS. Clostebol is the 4-chloro derivative of testosterone and the aim of the present study was the evaluation of clostebol sulfate metabolites in Caucasian population by LC-MS/MS technology. Clostebol was orally administered to four healthy Caucasian male volunteers, and excretion study urines were collected up to 31 days. Several analytical strategies (neutral loss scan, precursor ion scan and selected reaction monitoring acquisitions modes) were applied to detect sulfate metabolites in post-administration samples. Sixteen sulfate metabolites were detected, five of them having detectability times above 10 days (S1a, S2a, S3b, S3g and S4b). Interestingly, metabolite S1a could be detected up to the last collected sample of all excretion studies and it was characterized by LC-MS/MS and GC-MS as 4ξ-chloro-5α-androst-3β-ol-17-one 3β-sulfate. Thus, monitoring of S1a improves the detection time of clostebol misuse with respect to the commonly monitored metabolites, excreted in the glucuronide fraction. Importantly, this new metabolite can be incorporated into recently developed LC-MS/MS screening methods base on the direct detection of phase II metabolites.
dc.description.sponsorship The financial support received from the World Anti-Doping Agency WADA (12A21RV), Ministerio de Economía y Competividad (Gobierno de Espana)(Project number DEP2012-35612) and Generalitat de Catalunya (Consell Català de l’Esport, DIUE 2014 SGR 692) is gratefully acknowledged. Spanish Health National System is also acknowledged for O. J. Pozo contract (MS10/00576).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Science. 2016 Jun 1;1022:54-63
dc.rights © Elsevier. http://dx.doi.org/10.1016/j.jchromb.2016.03.028
dc.subject.other Anabolitzants
dc.subject.other Testosterona -- Metabolisme
dc.subject.other Metabòlits
dc.title Detection and characterization of clostebol sulfate metabolites in Caucasian population.
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.jchromb.2016.03.028
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/DEP2012-35612
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/acceptedVersion


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