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Selective constraints on protamine 2 in primates and rodents

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dc.contributor.author Lüke, Lena
dc.contributor.author Tourmente, Maximiliano
dc.contributor.author Dopazo, Hernán
dc.contributor.author Serra, François
dc.contributor.author Roldan, Eduardo R. S.
dc.date.accessioned 2016-06-03T14:09:09Z
dc.date.available 2016-06-03T14:09:09Z
dc.date.issued 2016
dc.identifier.citation Lüke L, Tourmente M, Dopazo H, Serra F, Roldan ER. Selective constraints on protamine 2 in primates and rodents. BMC evolutionary biology. 2016; 16: 21. DOI 10.1186/s12862-016-0588-1
dc.identifier.issn 1471-2148
dc.identifier.uri http://hdl.handle.net/10230/26820
dc.description.abstract BACKGROUND: Protamines are sperm nuclear proteins with a crucial role in chromatin condensation. Their function is strongly linked to sperm head morphology and male fertility. Protamines appear to be affected by a complex pattern of selective constraints. Previous studies showed that sexual selection affects protamine coding sequence and expression in rodents. Here we analyze selective constraints and post-copulatory sexual selection acting on protamine 2 (Prm2) gene sequences of 53 species of primates and rodents. We focused on possible differences in selective constraints between these two clades and on the two functional domains of PRM2 (cleaved- and mature-PRM2). We also assessed if and how changes in Prm2 coding sequence may affect sperm head dimensions. RESULTS: The domain of Prm2 that is cleaved off during binding to DNA (cleaved-Prm2) was found to be under purifying selection in both clades, whereas the domain that remains bound to DNA (mature-Prm2) was found to be positively selected in primates and under relaxed constraint in rodents. Changes in cleaved-Prm2 coding sequence are significantly correlated to sperm head width and elongation in rodents. Contrary to expectations, a significant effect of sexual selection was not found on either domain or clade. CONCLUSIONS: Mature-PRM2 may be free to evolve under less constraint due to the existence of PRM1 as a more conserved and functionally redundant copy. The cleaved-PRM2 domain seems to play an important role in sperm head shaping. However, sexual selection on its sequence may be difficult to detect until it is identified which sperm head phenotype (shape and size) confers advantages for sperm performance in different mammalian clades.
dc.description.sponsorship This work was supported by grant CGL2011-26341 from the Spanish Ministry of Economy and Competitiveness, a JAE-Predoc studentship from the Spanish National Research Council (CSIC) awarded to L.L., and a Juan de la Cierva postdoctoral fellowship awarded to M.T.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.ispartof BMC evolutionary biology. 2016; 16: 21
dc.rights © 2016 Lüke et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.other Semen
dc.title Selective constraints on protamine 2 in primates and rodents
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1186/s12862-016-0588-1
dc.subject.keyword Protamine
dc.subject.keyword Evolution
dc.subject.keyword Sexual selection
dc.subject.keyword Sperm competition
dc.subject.keyword Primates
dc.subject.keyword Rodents
dc.subject.keyword Spermatozoa
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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