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A genetic approach to study H2O2 scavenging in fission yeast--distinct roles of peroxiredoxin and catalase

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dc.contributor.author Paulo Mirasol, Esther, 1984-
dc.contributor.author García Santamarina, Sarela, 1978-
dc.contributor.author Calvo, Isabel A.
dc.contributor.author Carmona, Mercè
dc.contributor.author Boronat i Llop, Susanna, 1965-
dc.contributor.author Domènech, Alba
dc.contributor.author Ayté del Olmo, José
dc.contributor.author Hidalgo Hernando, Elena
dc.date.accessioned 2016-01-13T15:24:57Z
dc.date.available 2016-01-13T15:24:57Z
dc.date.issued 2014
dc.identifier.citation Paulo E, García-Santamarina S, Calvo IA, Carmona M, Boronat S, Domènech A et al. A genetic approach to study H2O2 scavenging in fission yeast--distinct roles of peroxiredoxin and catalase. Molecular microbiology. 2014; 92(2): 246-257. DOI 10.1111/mmi.12548
dc.identifier.issn 0950-382X
dc.identifier.uri http://hdl.handle.net/10230/25568
dc.description.abstract The main peroxiredoxin in Schizosaccharomyces pombe, Tpx1, is important to sustain aerobic growth, and cells lacking this protein are only able to grow on solid plates under anaerobic conditions. We have found that deletion of the gene coding for thioredoxin reductase, trr1, is a suppressor of the sensitivity to aerobic growth of Δtpx1 cells, so that cells lacking both proteins are able to grow on solid plates in the presence of oxygen. We have investigated this suppression effect, and determined that it depends on the presence of catalase, which is constitutively expressed in Δtrr1 cells in a transcription factor Pap1-dependent manner. A complete characterization of the repertoire of hydrogen peroxide scavenging activities in fission yeast suggests that Tpx1 is the only enzyme with sufficient sensitivity for peroxides and cellular abundance as to control the low levels produced during aerobic growth, catalase being the next barrier of detoxification when the steady-state levels of peroxides are increased in Δtpx1 cells. Gpx1, the only glutathione peroxidase encoded by the S. pombe genome, only has a minor secondary role when extracellular peroxides are added. Our study proposes non-overlapping roles for the different hydrogen peroxide scavenging activities of this eukaryotic organism
dc.description.sponsorship This work was supported by the Spanish Ministry of Economy and Competitiveness, Grants BFU2009-06933, BFU2012-32045, Plan E and FEDER; by/nthe Spanish program Consolider-Ingenio 2010 Grant CSD 2007-0020; and by SGR2009-195 from Generalitat de Catalunya (Spain) to E.H. E.H. and J.A. are recipients of ICREA Academia Awards (Generalitat de Catalunya).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Molecular microbiology. 2014; 92(2): 246-257
dc.rights This is the pre-peer reviewed version of the following article: Paulo E, García-Santamarina S, Calvo IA, Carmona M, Boronat S, Domènech A et al. A genetic approach to study H2O2 scavenging in fission yeast--distinct roles of peroxiredoxin and catalase. Molecular microbiology. 2014; 92(2): 246-257. DOI 10.1111/mmi.12548, which has been published in final form at http://dx.doi.org/10.1111/mmi.12548. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
dc.subject.other Schizosaccharomyces pombe -- Metabolisme
dc.subject.other Regulació genètica
dc.title A genetic approach to study H2O2 scavenging in fission yeast--distinct roles of peroxiredoxin and catalase
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1111/mmi.12548
dc.subject.keyword H2O2 scavenging
dc.subject.keyword Catalase
dc.subject.keyword Peroxiredoxin
dc.subject.keyword Fission yeast
dc.subject.keyword Pap1
dc.subject.keyword Glutathione
dc.subject.keyword 26 peroxidase
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-32045
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-06933
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/acceptedVersion


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