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mir-11 limits the proapoptotic function of its host gene, dE2f1

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dc.contributor.author Truscott, Mary
dc.contributor.author Islam, Abul, 1978-
dc.contributor.author López Bigas, Núria
dc.contributor.author Frolov, Maxim V.
dc.date.accessioned 2015-12-18T14:24:09Z
dc.date.available 2015-12-18T14:24:09Z
dc.date.issued 2011
dc.identifier.citation Truscott M, Islam AB, López-Bigas N, Frolov MV. mir-11 limits the proapoptotic function of its host gene, dE2f1. Genes & development. 2011; 25(17): 1820-1834. DOI 10.1101/gad.16947411
dc.identifier.issn 0890-9369
dc.identifier.uri http://hdl.handle.net/10230/25474
dc.description.abstract The E2F family of transcription factors regulates the expression of both genes associated with cell proliferation and genes that regulate cell death. The net outcome is dependent on cellular context and tissue environment. The mir-11 gene is located in the last intron of the Drosophila E2F1 homolog gene dE2f1, and its expression parallels that of dE2f1. Here, we investigated the role of miR-11 and found that miR-11 specifically modulated the proapoptotic function of its host gene, dE2f1. A mir-11 mutant was highly sensitive to dE2F1-dependent, DNA damage-induced apoptosis. Consistently, coexpression of miR-11 in transgenic animals suppressed dE2F1-induced apoptosis in multiple tissues, while exerting no effect on dE2F1-driven cell proliferation. Importantly, miR-11 repressed the expression of the proapoptotic genes reaper (rpr) and head involution defective (hid), which are directly regulated by dE2F1 upon DNA damage. In addition to rpr and hid, we identified a novel set of cell death genes that was also directly regulated by dE2F1 and miR-11. Thus, our data support a model in which the coexpression of miR-11 limits the proapoptotic function of its host gene, dE2f1, upon DNA damage by directly modulating a dE2F1-dependent apoptotic transcriptional program.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Cold Spring Harbor Laboratory Press (CSHL Press)
dc.relation.ispartof Genes & development. 2011; 25(17): 1820-1834
dc.rights © 2011. Genes and Development by Cold Spring Harbor Laboratory Press. Published version available at http://genesdev.cshlp.org/
dc.subject.other Drosòfila -- Genètica
dc.subject.other Factors de transcripció
dc.subject.other Cèl·lules HeLa
dc.title mir-11 limits the proapoptotic function of its host gene, dE2f1
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1101/gad.16947411
dc.subject.keyword Cell death
dc.subject.keyword Apoptosis
dc.subject.keyword E2F
dc.subject.keyword MicroRNA
dc.subject.keyword MiR-11
dc.subject.keyword DNA damage
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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