dc.contributor.author |
Ceballos Chávez, María |
dc.contributor.author |
Subtil Rodriguez, Alicia |
dc.contributor.author |
Giannopoulou, Eugenia G. |
dc.contributor.author |
Soronellas, Daniel |
dc.contributor.author |
Vázquez Chávez, Elena |
dc.contributor.author |
Vicent, Guillermo Pablo |
dc.contributor.author |
Elemento, Olivier |
dc.contributor.author |
Beato, Miguel |
dc.contributor.author |
Reyes, José C. |
dc.date.accessioned |
2015-11-19T13:28:05Z |
dc.date.available |
2015-11-19T13:28:05Z |
dc.date.issued |
2015 |
dc.identifier.citation |
Ceballos-Chávez M, Subtil-Rodríguez A, Giannopoulou EG, Soronellas D, Vázquez-Chávez E, Vicent GP et al. The chromatin Remodeler CHD8 is required for activation of progesterone receptor-dependent enhancers. PLoS genetics. 2015;11(4):e1005174. DOI: 10.1371/journal.pgen.1005174 |
dc.identifier.issn |
1553-7390 |
dc.identifier.uri |
http://hdl.handle.net/10230/25153 |
dc.description.abstract |
While the importance of gene enhancers in transcriptional regulation is well established, the mechanisms and the protein factors that determine enhancers activity have only recently begun to be unravelled. Recent studies have shown that progesterone receptor (PR) binds regions that display typical features of gene enhancers. Here, we show by ChIP-seq experiments that the chromatin remodeler CHD8 mostly binds promoters under proliferation conditions. However, upon progestin stimulation, CHD8 re-localizes to PR enhancers also enriched in p300 and H3K4me1. Consistently, CHD8 depletion severely impairs progestin-dependent gene regulation. CHD8 binding is PR-dependent but independent of the pioneering factor FOXA1. The SWI/SNF chromatin-remodelling complex is required for PR-dependent gene activation. Interestingly, we show that CHD8 interacts with the SWI/SNF complex and that depletion of BRG1 and BRM, the ATPases of SWI/SNF complex, impairs CHD8 recruitment. We also show that CHD8 is not required for H3K27 acetylation, but contributes to increase accessibility of the enhancer to DNaseI. Furthermore, CHD8 was required for RNAPII recruiting to the enhancers and for transcription of enhancer-derived RNAs (eRNAs). Taken together our data demonstrate that CHD8 is involved in late stages of PR enhancers activation. |
dc.format.mimetype |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Public Library of Science (PLoS) |
dc.relation.ispartof |
PLoS genetics. 2015;11(4):e1005174 |
dc.rights |
© 2015 Ceballos-Chávez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
dc.rights.uri |
http://creativecommons.org/licenses/by/4.0/ |
dc.subject.other |
Cromatina -- Estructura |
dc.title |
The chromatin Remodeler CHD8 is required for activation of progesterone receptor-dependent enhancers |
dc.type |
info:eu-repo/semantics/article |
dc.identifier.doi |
http://dx.doi.org/10.1371/journal.pgen.1005174 |
dc.rights.accessRights |
info:eu-repo/semantics/openAccess |
dc.type.version |
info:eu-repo/semantics/publishedVersion |