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The chromatin Remodeler CHD8 is required for activation of progesterone receptor-dependent enhancers

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dc.contributor.author Ceballos Chávez, María
dc.contributor.author Subtil Rodriguez, Alicia
dc.contributor.author Giannopoulou, Eugenia G.
dc.contributor.author Soronellas, Daniel
dc.contributor.author Vázquez Chávez, Elena
dc.contributor.author Vicent, Guillermo Pablo
dc.contributor.author Elemento, Olivier
dc.contributor.author Beato, Miguel
dc.contributor.author Reyes, José C.
dc.date.accessioned 2015-11-19T13:28:05Z
dc.date.available 2015-11-19T13:28:05Z
dc.date.issued 2015
dc.identifier.citation Ceballos-Chávez M, Subtil-Rodríguez A, Giannopoulou EG, Soronellas D, Vázquez-Chávez E, Vicent GP et al. The chromatin Remodeler CHD8 is required for activation of progesterone receptor-dependent enhancers. PLoS genetics. 2015;11(4):e1005174. DOI: 10.1371/journal.pgen.1005174
dc.identifier.issn 1553-7390
dc.identifier.uri http://hdl.handle.net/10230/25153
dc.description.abstract While the importance of gene enhancers in transcriptional regulation is well established, the mechanisms and the protein factors that determine enhancers activity have only recently begun to be unravelled. Recent studies have shown that progesterone receptor (PR) binds regions that display typical features of gene enhancers. Here, we show by ChIP-seq experiments that the chromatin remodeler CHD8 mostly binds promoters under proliferation conditions. However, upon progestin stimulation, CHD8 re-localizes to PR enhancers also enriched in p300 and H3K4me1. Consistently, CHD8 depletion severely impairs progestin-dependent gene regulation. CHD8 binding is PR-dependent but independent of the pioneering factor FOXA1. The SWI/SNF chromatin-remodelling complex is required for PR-dependent gene activation. Interestingly, we show that CHD8 interacts with the SWI/SNF complex and that depletion of BRG1 and BRM, the ATPases of SWI/SNF complex, impairs CHD8 recruitment. We also show that CHD8 is not required for H3K27 acetylation, but contributes to increase accessibility of the enhancer to DNaseI. Furthermore, CHD8 was required for RNAPII recruiting to the enhancers and for transcription of enhancer-derived RNAs (eRNAs). Taken together our data demonstrate that CHD8 is involved in late stages of PR enhancers activation.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PLoS genetics. 2015;11(4):e1005174
dc.rights © 2015 Ceballos-Chávez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.other Cromatina -- Estructura
dc.title The chromatin Remodeler CHD8 is required for activation of progesterone receptor-dependent enhancers
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1371/journal.pgen.1005174
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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