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Enhanced transcriptome maps from multiple mouse tissues reveal evolutionary constraint in gene expression

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dc.contributor.author Pervouchine, Dmitri D.
dc.contributor.author Djebali, Sarah
dc.contributor.author Breschi, Alessandra, 1988-
dc.contributor.author Prieto Barja, Pablo, 1986-
dc.contributor.author Lagarde, Julien
dc.contributor.author Bussotti, Giovanni, 1983-
dc.contributor.author Monlong, Jean
dc.contributor.author Notredame, Cedric
dc.contributor.author Guigó Serra, Roderic
dc.contributor.author Gingeras, Thomas R.
dc.date.accessioned 2015-11-16T15:47:14Z
dc.date.available 2015-11-16T15:47:14Z
dc.date.issued 2015
dc.identifier.citation Pervouchine DD, Djebali S, Breschi A, Davis CA, Barja PP, Dobin A et al. Enhanced transcriptome maps from multiple mouse tissues reveal evolutionary constraint in gene expression. Nature communications. 2015; 6: 5903. DOI 10.1038/ncomms6903
dc.identifier.issn 2041-1723
dc.identifier.uri http://hdl.handle.net/10230/25109
dc.description.abstract Mice have been a long-standing model for human biology and disease. Here we characterize, by RNA sequencing, the transcriptional profiles of a large and heterogeneous collection of mouse tissues, augmenting the mouse transcriptome with thousands of novel transcript candidates. Comparison with transcriptome profiles in human cell lines reveals substantial conservation of transcriptional programmes, and uncovers a distinct class of genes with levels of expression that have been constrained early in vertebrate evolution. This core set of genes captures a substantial fraction of the transcriptional output of mammalian cells, and participates in basic functional and structural housekeeping processes common to all cell types. Perturbation of these constrained genes is associated with significant phenotypes including embryonic lethality and cancer. Evolutionary constraint in gene expression levels is not reflected in the conservation of the genomic sequences, but is associated with conserved epigenetic marking, as well as with characteristic post-transcriptional regulatory programme, in which sub-cellular localization and alternative splicing play comparatively large roles.
dc.description.sponsorship This work was supported by the National Human Genome Research Institute (NHGRI) grants number 1U54HG007004-1, U41HG007000, U01HG004695, U54HG004555 and U41HG007234, by the Spanish Plan Nacional grants number BIO2011-26205 and BFU2011-28575, the ERC grant number 294653, LaCaixa and the EU-FP7 quantomics project.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Nature Pub. Group
dc.relation.ispartof Nature communications. 2015; 6: 5903
dc.rights © 2015 Macmillan Publishers Limited. All rights reserved. This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject.other Ratolins -- Genètica
dc.subject.other Regulació genètica
dc.title Enhanced transcriptome maps from multiple mouse tissues reveal evolutionary constraint in gene expression
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1038/ncomms6903
dc.relation.projectID info:eu-repo/grantAgreement/EC/ERC/294653
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2011-26205
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2011-28575
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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