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Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium

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dc.contributor.author Lluch-Senar, Maria 1982-
dc.contributor.author Delgado Blanco, Javier
dc.contributor.author Chen, Wei-Hua
dc.contributor.author Lloréns Rico, Verónica, 1989-
dc.contributor.author O’Reilly, Francis J.
dc.contributor.author Wodke, Judith A.H.
dc.contributor.author Unal, E. Besray
dc.contributor.author Yus, Eva
dc.contributor.author Martínez, Sira
dc.contributor.author Nichols, Robert J.
dc.contributor.author Ferrar, Tony
dc.contributor.author Vivancos Prellezo, Ana
dc.contributor.author Schmeisky, Arne
dc.contributor.author Stülke, Jörg
dc.contributor.author Noort, Vera van
dc.contributor.author Gavin, Anne-Claude
dc.contributor.author Bork, Peer
dc.contributor.author Serrano Pubull, Luis, 1982-
dc.date.accessioned 2015-11-16T14:33:37Z
dc.date.available 2015-11-16T14:33:37Z
dc.date.issued 2015
dc.identifier.citation Lluch-Senar M, Delgado J, Chen WH, Lloréns-Rico V, O'Reilly FJ, Wodke JA et al. Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium. Molecular systems biology. 2015;11(1):780. DOI: 10.15252/msb.20145558
dc.identifier.issn 1744-4292
dc.identifier.uri http://hdl.handle.net/10230/25104
dc.description.abstract Identifying all essential genomic components is critical for the assembly of minimal artificial life. In the genome-reduced bacterium Mycoplasma pneumoniae, we found that small ORFs (smORFs; < 100 residues), accounting for 10% of all ORFs, are the most frequently essential genomic components (53%), followed by conventional ORFs (49%). Essentiality of smORFs may be explained by their function as members of protein and/or DNA/RNA complexes. In larger proteins, essentiality applied to individual domains and not entire proteins, a notion we could confirm by expression of truncated domains. The fraction of essential non-coding RNAs (ncRNAs) non-overlapping with essential genes is 5% higher than of non-transcribed regions (0.9%), pointing to the important functions of the former. We found that the minimal essential genome is comprised of 33% (269,410 bp) of the M. pneumoniae genome. Our data highlight an unexpected hidden layer of smORFs with essential functions, as well as non-coding regions, thus changing the focus when aiming to define the minimal essential genome.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Wiley Blackwell
dc.relation.ispartof Molecular systems biology. 2015;11(1):780
dc.rights © 2015 The Authors. Published under the terms of the CC BY 4.0 license. This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
dc.subject.other RNA no missatgers
dc.subject.other Interaccions RNA-proteïna
dc.title Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.15252/msb.20145558
dc.subject.keyword Minimal genome
dc.subject.keyword Non-coding RNAs
dc.subject.keyword Small proteins
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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