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A computational clonal analysis of the developing mouse limb bud

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dc.contributor.author Marcon, Luciano, 1983-
dc.contributor.author Arqués, Carlos G.
dc.contributor.author Torres, Miguel S.
dc.contributor.author Sharpe, James
dc.date.accessioned 2015-07-22T10:19:44Z
dc.date.available 2015-07-22T10:19:44Z
dc.date.issued 2011
dc.identifier.citation Marcon L, Arqués CG, Torres MS, Sharpe J. A computational clonal analysis of/nthe developing mouse limb bud. PLoS Comput Biol. 2011 Feb 10;7(2):e1001071. DOI: 10.1371/journal.pcbi.1001071
dc.identifier.issn 1553-734X
dc.identifier.uri http://hdl.handle.net/10230/24628
dc.description.abstract A comprehensive spatio-temporal description of the tissue movements underlying organogenesis would be an extremely useful resource to developmental biology. Clonal analysis and fate mappings are popular experiments to study tissue movement during morphogenesis. Such experiments allow cell populations to be labeled at an early stage of development and to follow their spatial evolution over time. However, disentangling the cumulative effects of the multiple events responsible for the expansion of the labeled cell population is not always straightforward. To overcome this problem, we develop a novel computational method that combines accurate quantification of 2D limb bud morphologies and growth modeling to analyze mouse clonal data of early limb development. Firstly, we explore various tissue movements that match experimental limb bud shape changes. Secondly, by comparing computational clones with newly generated mouse clonal data we are able to choose and characterize the tissue movement map that better matches experimental data. Our computational analysis produces for the first time a two dimensional model of limb growth based on experimental data that can be used to better characterize limb tissue movement in space and time. The model shows that the distribution and shapes of clones can be described as a combination of anisotropic growth with isotropic cell mixing, without the need for lineage compartmentalization along the AP and PD axis. Lastly, we show that this comprehensive description can be used to reassess spatio-temporal gene regulations taking tissue movement into account and to investigate PD patterning hypothesis.
dc.description.sponsorship The work was funded by the MADRICEL grant from the Madrid Regional Government (S-SAL-0190-2006)
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PLoS Comput Biol. 2011 Feb 10;7(2):e1001071
dc.rights © 2011 Marcon et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject.other Clonatge
dc.subject.other Ratolins (Animals de laboratori)
dc.title A computational clonal analysis of the developing mouse limb bud
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/ 10.1371/journal.pcbi.1001071
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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