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Secondary enhancers synergise with primary enhancers to guarantee fine-tuned muscle gene expression

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dc.contributor.author Guerrero, Lucia
dc.contributor.author Marco Ferreres, Raquel
dc.contributor.author Serrano, Antonio L.
dc.contributor.author Arredondo, Juan Jose
dc.contributor.author Cervera, Margarita
dc.date.accessioned 2015-06-04T06:56:57Z
dc.date.available 2015-06-04T06:56:57Z
dc.date.issued 2010
dc.identifier.citation Guerrero L, Marco-Ferreres R, Serrano AL, Arredondo JJ, Cervera M. Secondary enhancers synergise with primary enhancers to guarantee fine-tuned muscle gene expression. Developmental Biology. 2010;337(1):16-28. DOI: 10.1016/j.ydbio.2009.10.006
dc.identifier.issn 0012-1606
dc.identifier.uri http://hdl.handle.net/10230/23728
dc.description.abstract Although tight quantitative control of gene expression is required to ensure that organs and tissues function correctly, the transcriptional mechanisms underlying this process still remain poorly understood. Here, we describe novel and evolutionary conserved secondary enhancers that are needed for the regulation of the expression of Troponin I genes. Secondary enhancers are silent when tested individually in electroporated muscles but interact with the primary enhancers and are required to precisely control the appropriate timing, the tissue and fibre specificity, and the quantitative expression of these genes during muscle differentiation. Synergism is completely dependent of the fully conserved MEF2 site present on the primary enhancers core of skeletal muscle Troponin I genes. Thus, while each of these paired enhancers has a different function, the concerted action of both is crucial to recapitulate endogenous gene expression. Through comparative genomics, we predict that this mechanism has also arisen in other mammalian muscle genes. Our results reveal the existence of a novel mechanism, conserved from flies to mammals, to fine-tune gene expression in each muscle and probably other tissues
dc.description.sponsorship This research was supported by grants BFU2004-05384, BFU2007-61711BMC from the DGICYT (Spanish Ministry of Education, Culture and Sports), FIS-PS09/01267, Marato-TV3 and by Fundación Mutua Madrileña
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof Developmental Biology. 2010;337(1):16-28
dc.rights © Elsevier This is the published version of an article http://dx.doi.org/10.1016/j.ydbio.2009.10.006 that appeared in the journal Developmental Biology. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-license
dc.subject.other Ratolins (Animals de laboratori)
dc.subject.other Genètica animal
dc.title Secondary enhancers synergise with primary enhancers to guarantee fine-tuned muscle gene expression
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.ydbio.2009.10.006
dc.subject.keyword Paired enhancers
dc.subject.keyword Secondary enhancers
dc.subject.keyword Fine-tuning gene-transcription control
dc.subject.keyword Global transcription regulation
dc.subject.keyword Mouse fast, slow and cardiac Troponin I genes
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/BFU2004-05384
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/BFU2007-61711
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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